Niels H Søe1, Nina Vendel Jensen2, Asger Lundorff Jensen3, Janne Koch4, Steen Seier Poulsen5, Gerald B Pier6, Helle Krogh Johansen7,8. 1. Hand Section, Department of Orthopaedics, Herlev and Gentofte University Hospital, Hellerup, Denmark Niels.Soee.Nielsen@regionh.dk. 2. Department of Anaesthesiology, Intensive Care and Operations, Herlev and Gentofte University Hospital, Hellerup, Denmark. 3. Biochemical Department, Faculty of Life Science, University of Copenhagen, Copenhagen, Denmark. 4. Department of Experimental Medicine, Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark. 5. Biomedical Department, Panum Institute, University of Copenhagen, Copenhagen, Denmark. 6. Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, U.S.A. 7. Department of Clinical Microbiology, Rigshospitalet, Copenhagen University, Copenhagen, Denmark. 8. The Novo Nordisk Foundation, Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
Abstract
BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization. MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 103 S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus. RESULTS/ CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica+) but not ica- S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica+ or ica- S. aureus, suggesting a lack of specificity in this antiserum. Copyright
BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization. MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 103 S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharidepoly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus. RESULTS/ CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica+) but not ica- S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica+ or ica- S. aureus, suggesting a lack of specificity in this antiserum. Copyright
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