Literature DB >> 28058684

Development and investigation of recombinant immunotoxin protein 4D5scFv-mCherry-PE(40).

O N Shilova1, E A Souslova2, A M Pilunov2, S M Deyev2,3, R V Petrov2.   

Abstract

Development of agents for theranostics implies combining the targeting module, the effector module, and the detection module within the same complex or recombinant protein. We have constructed, isolated, and characterized the 4D5scFv-mCherry-PE(40) protein, which exhibits fluorescent properties and specifically binds to cancer cells expressing the HER2 receptor and reduces their viability. The ability of the obtained targeted antitumor agent 4D5scFv-mCherry-PE(40) to selectively stain the HER2-positive cells and its highly selective cytotoxicity against these cells make the obtained targeted recombinant protein 4D5scFv-mCherry-PE(40) a promising theranostic agent for the diagnostics and therapy of HER2-positive human tumors.

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Year:  2017        PMID： 28058684     DOI： 10.1134/S160767291606020X

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  8 in total

Review 1.  Fluorescent proteins and their applications in imaging living cells and tissues.

Authors:  Dmitriy M Chudakov; Mikhail V Matz; Sergey Lukyanov; Konstantin A Lukyanov
Journal:  Physiol Rev       Date:  2010-07       Impact factor: 37.312

2.  HER-2/neu gene amplification characterized by fluorescence in situ hybridization: poor prognosis in node-negative breast carcinomas.

Authors:  M F Press; L Bernstein; P A Thomas; L F Meisner; J Y Zhou; Y Ma; G Hung; R A Robinson; C Harris; A El-Naggar; D J Slamon; R N Phillips; J S Ross; S R Wolman; K J Flom
Journal:  J Clin Oncol       Date:  1997-08       Impact factor: 44.544

3.  Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

Authors:  Nathan C Shaner; Robert E Campbell; Paul A Steinbach; Ben N G Giepmans; Amy E Palmer; Roger Y Tsien
Journal:  Nat Biotechnol       Date:  2004-11-21       Impact factor: 54.908

4.  Highly specific hybrid protein DARPin-mCherry for fluorescent visualization of cells overexpressing tumor marker HER2/neu.

Authors:  K E Mironova; O N Chernykh; A V Ryabova; O A Stremovskiy; G M Proshkina; S M Deyev
Journal:  Biochemistry (Mosc)       Date:  2014-12       Impact factor: 2.487

5.  Photoactivatable mCherry for high-resolution two-color fluorescence microscopy.

Authors:  Fedor V Subach; George H Patterson; Suliana Manley; Jennifer M Gillette; Jennifer Lippincott-Schwartz; Vladislav V Verkhusha
Journal:  Nat Methods       Date:  2009-01-25       Impact factor: 28.547

6.  Modern Technologies for Creating Synthetic Antibodies for Clinical application.

Authors:  S M Deyev; E N Lebedenko
Journal:  Acta Naturae       Date:  2009-04       Impact factor: 1.845

Review 7.  A guide to taming a toxin--recombinant immunotoxins constructed from Pseudomonas exotoxin A for the treatment of cancer.

Authors:  John E Weldon; Ira Pastan
Journal:  FEBS J       Date:  2011-06-02       Impact factor: 5.622

8.  Genetically encoded immunophotosensitizer 4D5scFv-miniSOG is a highly selective agent for targeted photokilling of tumor cells in vitro.

Authors:  Kristina E Mironova; Galina M Proshkina; Anastasiya V Ryabova; Oleg A Stremovskiy; Sergey A Lukyanov; Rem V Petrov; Sergey M Deyev
Journal:  Theranostics       Date:  2013-10-12       Impact factor: 11.556
  8 in total

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