Xia Chen1, Xiaofei Zhao1, Jie Che1, Yanwen Xiong1, Yanmei Xu1, Lifeng Zhang1, Ruiting Lan2, Lining Xia3, Timothy R Walsh4, Jianguo Xu1, Jinxing Lu1, Juan Li1. 1. State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, PR China. 2. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia. 3. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, PR China. 4. Department of Medical Microbiology, School of Medicine, Cardiff University, Cardiff, UK.
Abstract
PURPOSE: A recently identified colistin resistance gene, mcr-1, has been reported in many countries. In this study, we established a new real-time PCR method to detect it. METHODOLOGY: We used a real-time PCR method to detect the mcr-1 gene in a variety of isolates and faecal samples from 20 provinces and municipal cities in China. RESULTS: Of the 2330 isolates (from 10 species) screened, 54 (2.3 %) isolates were positive for mcr-1. All of the mcr-1-positive isolates that were identified belonged to Escherichia coli strains, among which 9, 1, and 44 were identified as enteropathogenic E. coli, enteroadherent E. coli, and non-pathogenic E. coli, respectively. The majority of the mcr-1-positive isolates were obtained from farm animals from eight provinces and municipal cities across China. A total of 337 faecal samples, including 229 human and 108 pet animal faecal samples, were also screened for the mcr-1 gene. Of the 337 samples analyzed, six and eight human and pet animal faecal samples were positive for the mcr-1 gene, respectively. CONCLUSION: The data demonstrate that the mcr-1 gene is highly prevalent in human and animal populations in China. This occurrence suggests that active surveillance of the mcr-1 gene is imperative in curtailing its spread.
PURPOSE: A recently identified colistin resistance gene, mcr-1, has been reported in many countries. In this study, we established a new real-time PCR method to detect it. METHODOLOGY: We used a real-time PCR method to detect the mcr-1 gene in a variety of isolates and faecal samples from 20 provinces and municipal cities in China. RESULTS: Of the 2330 isolates (from 10 species) screened, 54 (2.3 %) isolates were positive for mcr-1. All of the mcr-1-positive isolates that were identified belonged to Escherichia coli strains, among which 9, 1, and 44 were identified as enteropathogenic E. coli, enteroadherent E. coli, and non-pathogenic E. coli, respectively. The majority of the mcr-1-positive isolates were obtained from farm animals from eight provinces and municipal cities across China. A total of 337 faecal samples, including 229 human and 108 pet animal faecal samples, were also screened for the mcr-1 gene. Of the 337 samples analyzed, six and eight human and pet animal faecal samples were positive for the mcr-1 gene, respectively. CONCLUSION: The data demonstrate that the mcr-1 gene is highly prevalent in human and animal populations in China. This occurrence suggests that active surveillance of the mcr-1 gene is imperative in curtailing its spread.
Authors: Pedro Miguela-Villoldo; Miguel A Moreno; David Rodríguez-Lázaro; Alejandro Gallardo; Marta Hernández; Tania Serrano; José L Sáez; Cristina de Frutos; Montserrat Agüero; Alberto Quesada; Lucas Domínguez; María Ugarte-Ruiz Journal: Porcine Health Manag Date: 2022-03-17
Authors: Carlos Bastidas-Caldes; Jacobus H de Waard; María Soledad Salgado; María José Villacís; Marco Coral-Almeida; Yoshimasa Yamamoto; Manuel Calvopiña Journal: Pathogens Date: 2022-06-08
Authors: Madubuike Umunna Anyanwu; Ishmael Festus Jaja; Obichukwu Chisom Nwobi Journal: Int J Environ Res Public Health Date: 2020-02-06 Impact factor: 3.390