Rachel K Marvin1, Muncharie B Saepoo2, Simiao Ye3, Donald B White3, Rong Liu3, Kenneth Hensley4, Paul Rega5, Viviane Kazan5, David R Giovannucci2, Dragan Isailovic1. 1. a Department of Chemistry and Biochemistry , University of Toledo , Toledo , OH , USA. 2. b Department of Neuroscience , University of Toledo , Toledo , OH , USA. 3. c Department of Mathematics and Statistics , University of Toledo , Toledo , OH , USA. 4. d Department of Pathology , University of Toledo, Toledo , OH, USA. 5. e Department of Emergency Medicine , University of Toledo , Toledo , OH , USA.
Abstract
CONTEXT: Quantitative changes of salivary proteins due to acute stress were detected. OBJECTIVE: To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations. MATERIALS AND METHODS: Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry. RESULTS: Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress. DISCUSSION AND CONCLUSION: Alpha-amylase and cystatins are potential salivary markers of acute stress, but further validation should be performed using larger sample populations.
CONTEXT: Quantitative changes of salivary proteins due to acute stress were detected. OBJECTIVE: To explore protein markers of stress in saliva of eight medical residents who performed emergency medicine simulations. MATERIALS AND METHODS: Saliva was collected before the simulations, after the simulations, and following morning upon waking. Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), identified by mass spectrometry (MS), and relatively quantified by densitometry. RESULTS:Salivary alpha-amylase and S-type cystatins significantly increased, while the ∼26 kDa and low-molecular weight (MW) (<10 kDa) SDS-PAGE bands exhibited changes after stress. DISCUSSION AND CONCLUSION: Alpha-amylase and cystatins are potential salivary markers of acute stress, but further validation should be performed using larger sample populations.
Entities:
Keywords:
Saliva; amylase; cystatins; gel electrophoresis; mass spectrometry; stress
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