| Literature DB >> 28043388 |
Sandra Valéria Inácio1, Giovanni Widmer2, Roberta Lomonte Lemos de Brito3, Anaiza Simão Zucatto4, Monally Conceição Costa de Aquino5, Bruno César Miranda Oliveira6, Alex Akira Nakamura7, Luiz da Silveira Neto8, João Gabriel Balizardo Carvalho9, Jancarlo Ferreira Gomes10, Marcelo Vasconcelos Meireles11, Katia Denise Saraiva Bresciani12.
Abstract
The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.Entities:
Keywords: Equine; Molecular biology; Parasite
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Year: 2016 PMID： 28043388 DOI： 10.1016/j.vetpar.2016.11.021
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738