Literature DB >> 28039562

Long Shelf Life of a Lyophilized DNA Aptamer Beacon Assay.

John G Bruno1.   

Abstract

An aptamer beacon previously developed to detect C-telopeptide (CTx) from human bone collagen breakdown was lyophilized and shown to give a "lights on" concentration-dependent spectral fluorescence response essentially identical to that of the fresh reagent despite storage in a dark dry environment for the past 5.5 years.

Entities:  

Keywords:  Aptamer; Beacon; DNA; Freeze-dried; Lyophilization; Shelf life

Mesh:

Substances:

Year:  2016        PMID: 28039562     DOI: 10.1007/s10895-016-2014-x

Source DB:  PubMed          Journal:  J Fluoresc        ISSN: 1053-0509            Impact factor:   2.217


  5 in total

1.  Stability of fluorescent antibody conjugates stored under various conditions.

Authors:  J H Green; S B Gray; W K Harrell
Journal:  J Clin Microbiol       Date:  1976-01       Impact factor: 5.948

2.  Long term stability of lyophilized plasmid DNA pDERMATT.

Authors:  Iris van der Heijden; Jos H Beijnen; Bastiaan Nuijen
Journal:  Int J Pharm       Date:  2013-06-19       Impact factor: 5.875

3.  Shelf life of fluorescent treponemal antibody-absorption test reagents.

Authors:  M E Roberts; J N Miller; T C Pringle; G F Binnings
Journal:  J Bacteriol       Date:  1968-11       Impact factor: 3.490

4.  DNA aptamer beacon assay for C-telopeptide and handheld fluorometer to monitor bone resorption.

Authors:  John Gordon Bruno; Maria P Carrillo; Taylor Phillips; Douglas Hanson; Jonathan A Bohmann
Journal:  J Fluoresc       Date:  2011-06-04       Impact factor: 2.217

5.  Naked plasmid DNA formulation: effect of different disaccharides on stability after lyophilisation.

Authors:  Susanne G L Quaak; John B A G Haanen; Jos H Beijnen; Bastiaan Nuijen
Journal:  AAPS PharmSciTech       Date:  2010-03-04       Impact factor: 3.246

  5 in total
  1 in total

1.  Functional Blockade of E-Selectin in Tumor-Associated Vessels Enhances Anti-Tumor Effect of Doxorubicin in Breast Cancer.

Authors:  Yoshihiro Morita; Macall Leslie; Hiroyasu Kameyama; Ganesh L R Lokesh; Norihisa Ichimura; Rachel Davis; Natalie Hills; Nafis Hasan; Roy Zhang; Yuji Kondo; David G Gorenstein; David E Volk; Inna Chervoneva; Hallgeir Rui; Takemi Tanaka
Journal:  Cancers (Basel)       Date:  2020-03-19       Impact factor: 6.639

  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.