Literature DB >> 2803305

Comparative activity of alpha- and beta-anomeric oligonucleotides on rabbit beta globin synthesis: inhibitory effect of cap targeted alpha-oligonucleotides.

J R Bertrand1, B Rayner, J L Imbach, C Paoletti, C Malvy.   

Abstract

Alpha-anomeric oligonucleotides are resistant to nucleases and display parallel annealing to RNA complementary sequences. We compared the effect of alpha- and beta-oligonucleotides targeted against various mRNA regions on the rabbit beta globin in vitro synthesis. In order to determine the role of RNase H, experiments were performed in both rabbit reticulocyte lysate and wheat germ extract. As expected beta-oligonucleotides were found more efficient in wheat germ extract which is rich in RNase H activity and alpha-oligonucleotide targeted against the initiation codon or downstream had no effect because they do not induce mRNA cleavage by RNase H. However, we report, for the first time, a specific translation inhibition by alpha-oligonucleotides. This occurs provided they are targeted against the cap region in 5' of the mRNA.

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Year:  1989        PMID： 2803305     DOI： 10.1016/0006-291x(89)91719-1

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  13 in total

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Authors:  J M Aramini; A Mujeeb; N B Ulyanov; M W Germann
Journal:  J Biomol NMR       Date:  2000-12       Impact factor: 2.835

4.  In vitro inhibition of hepatitis C virus gene expression by chemically modified antisense oligodeoxynucleotides.

Authors:  O Vidalin; M E Major; B Rayner; J L Imbach; C Trépo; G Inchauspé
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5.  Effects of antisense oligodeoxynucleotide hybridization on in vitro translation of potato virus Y RNA.

Authors:  C Levis; M Tronchet; M Meyer; J Albouy; S Astier-Manifacier
Journal:  Virus Genes       Date:  1992-01       Impact factor: 2.332

6.  Synthesis and base-pairing properties of the nuclease-resistant alpha-anomeric dodecaribonucleotide alpha-[r(UCUUAACCCACA)].

Authors:  F Debart; B Rayner; G Degols; J L Imbach
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

7.  Antisense phosphorothioate oligonucleotides: selective killing of the intracellular parasite Leishmania amazonensis.

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Journal:  Proc Natl Acad Sci U S A       Date:  1994-08-16       Impact factor: 11.205

8.  Structure and conformation in solution of the parallel-stranded hybrid alpha-d(CGCAATTCGC).beta-d(GCGTTAAGCG) by high-resolution 2D NMR.

Authors:  W H Gmeiner; B Rayner; F Morvan; J L Imbach; J W Lown
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9.  Target-specific arrest of mRNA translation by antisense 2'-O-alkyloligoribonucleotides.

Authors:  H E Johansson; G J Belsham; B S Sproat; M W Hentze
Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

10.  Sequence-specific interaction of alpha-beta-anomeric double-stranded DNA with the p50 subunit of NF kappa B: application to the decoy approach.

Authors:  H Tanaka; P Vickart; J R Bertrand; B Rayner; F Morvan; J L Imbach; D Paulin; C Malvy
Journal:  Nucleic Acids Res       Date:  1994-08-11       Impact factor: 16.971
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