Literature DB >> 28031195

attI1-Located Small Open Reading Frames ORF-17 and ORF-11 in a Class 1 Integron Affect Expression of a Gene Cassette Possessing a Canonical Shine-Dalgarno Sequence.

Costas C Papagiannitsis1, Leonidas S Tzouvelekis2, Eva Tzelepi1, Vivi Miriagou3.   

Abstract

By searching the Integrall integron and GenBank databases, a novel open reading frame (ORF) of 51 nucleotides (nts) (ORF-17) overlapping the previously described ORF-11 was identified within the attI1 site in virtually all class 1 integrons. Using a set of isogenic plasmid constructs carrying a single gene cassette (blaGES-1) and possessing a canonical translation initiation region, we found that ORF-17 contributes to GES-1 expression.
Copyright © 2017 American Society for Microbiology.

Entities:  

Keywords:  attI1 sequence; class 1 integrons; expression; gene cassettes

Mesh:

Substances:

Year:  2017        PMID: 28031195      PMCID: PMC5328530          DOI: 10.1128/AAC.02070-16

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


  9 in total

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3.  Definition of the attI1 site of class 1 integrons.

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Authors:  Costas C Papagiannitsis; Leonidas S Tzouvelekis; Vivi Miriagou
Journal:  Antimicrob Agents Chemother       Date:  2008-11-10       Impact factor: 5.191

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