Ying Sun1, Chun-Mei Lu2, Zhen Song3, Ke-Ke Xu3, Shu-Bin Wu3, Zhi-Jian Li3. 1. Department of Ophthalmology, First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang Province, China; Department of Ophthalmology, Second Hospital of Heilongjiang Province, Harbin 150001, Heilongjiang Province, China. 2. Department of Physiology, Harbin Medical University, Harbin 150001, Heilongjiang Province, China. 3. Department of Ophthalmology, First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
Abstract
AIM: To determine the role of microRNA (miRNA)-29a and miRNA-29c in the regulation of apoptosis in early rat diabetic cataract formation. METHODS: Streptozotocin (STZ)-induced diabetic Sprague-Dawley (SD) rats were used in the study. The expression level of miRNA-29a, miRNA-29c, and BCL2-modifying factor (BMF) in lens epithelial cells (LECs) samples were measured using quantitative real-time polymerase chain reaction. Prediction algorithms of miRanda, TargetScan 6.2, and mirRDB to perform a miRNA gene network analysis were used for the potential miRNA-29a and miRNA-29c targets. RESULTS: The miRNA-29a and miRNA-29c expression levels were all significantly lower in the control group compared to the 2 and 4wk diabetic samples (P<0.01). The network analysis indicated that one miRNA-29a and miRNA-29c targets was BMF. There was significantly higher expression of BMF mRNA compared to the normal controls (P<0.01). CONCLUSION: Apoptosis occurs in rat LECs following high blood glucose exposure. It is likely that apoptosis during diabetic cataract formation involves the decreased expression of miRNA-29a and miRNA-29c and the increased expression of BMF.
AIM: To determine the role of microRNA (miRNA)-29a and miRNA-29c in the regulation of apoptosis in early ratdiabetic cataract formation. METHODS:Streptozotocin (STZ)-induced diabetic Sprague-Dawley (SD) rats were used in the study. The expression level of miRNA-29a, miRNA-29c, and BCL2-modifying factor (BMF) in lens epithelial cells (LECs) samples were measured using quantitative real-time polymerase chain reaction. Prediction algorithms of miRanda, TargetScan 6.2, and mirRDB to perform a miRNA gene network analysis were used for the potential miRNA-29a and miRNA-29c targets. RESULTS: The miRNA-29a and miRNA-29c expression levels were all significantly lower in the control group compared to the 2 and 4wk diabetic samples (P<0.01). The network analysis indicated that one miRNA-29a and miRNA-29c targets was BMF. There was significantly higher expression of BMF mRNA compared to the normal controls (P<0.01). CONCLUSION: Apoptosis occurs in rat LECs following high blood glucose exposure. It is likely that apoptosis during diabetic cataract formation involves the decreased expression of miRNA-29a and miRNA-29c and the increased expression of BMF.
Authors: W C Li; J R Kuszak; K Dunn; R R Wang; W Ma; G M Wang; A Spector; M Leib; A M Cotliar; M Weiss Journal: J Cell Biol Date: 1995-07 Impact factor: 10.539