| Literature DB >> 28003349 |
James Pettitt1, Elizabeth Higgs2, Mosoka Fallah3, Martha Nason4, Eric Stavale1, Jonathan Marchand1, Cavan Reilly5, Kenneth Jensen1, Bonnie Dighero-Kemp1, Kaylie Tuznik1, James Logue1, Fatorma Bolay6, Lisa Hensley1.
Abstract
Recent studies have suggested that Ebola virus (EBOV) ribonucleic acid (RNA) potentially present in the semen of a large number of survivors of Ebola virus disease (EVD) in Western Africa may contribute to sexual transmission of EVD and generate new clusters of cases in regions previously declared EVD-free. These findings drive the immediate need for a reliable, rapid, user-friendly assay for detection of EBOV RNA in semen that is deployable to multiple sites across Western Africa. In this study, we optimized the Xpert EBOV assay for semen samples by adding dithiothreitol. Compared to the assays currently in use in Liberia (including Ebola Zaire Target 1, major groove binder real-time-polymerase chain reaction assays, and original Xpert EBOV assay), the modified Xpert EBOV assay demonstrated greater sensitivity than the comparator assays. Thus, the modified Xpert EBOV assay is optimal for large-scale monitoring of EBOV RNA persistence in male survivors. Published by Oxford University Press for the Infectious Diseases Society of America 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.Entities:
Keywords: Diagnostics Test; Ebola virus; RNA; Semen; Survivors; Viral persistence; filoviruses
Mesh：
Substances：
Year: 2017 PMID： 28003349 PMCID： PMC6075475 DOI： 10.1093/infdis/jiw599
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226