OBJECTIVE: Lipopolysaccharide binding protein (LBP) is part of a family of structurally and functionally related lipid transfer proteins. This study aimed to investigate the associations of LBP with the lipid composition of human adipose tissue. METHODS: Lipidomic analysis was performed in whole adipose tissue. To validate the associations found, LBP was knocked down (KD) in 3T3-L1 adipocytes, and lipidomic profile was evaluated by nontargeted lipidomics. RESULTS: LBP gene expression was negatively associated with phosphatidylcholine, phosphatidylserine, and sphingomyelin relative abundance in vivo. LBP expression was also decreased in those samples with the highest docosahexanoic content, implicated in the resolution of inflammation. The KD of LBP (by ∼70%) led to sharp changes in the lipidome of adipocytes. Of note, specific plasmalogen species PE(O-16:0/22:5), PE(38:2), odd chain glycerolipids, and cholesteryl linoleate were upregulated by LBP KD. In contrast, GM3 gangliosides, several ceramides, a triacylglycerol potentially containing arachidonate, and cholesteryl palmitate were downregulated by LBP KD. CONCLUSIONS: LBP seems to play a role in the regulation of lipid composition of adipose tissue linked to resilience to inflammation.
OBJECTIVE:Lipopolysaccharide binding protein (LBP) is part of a family of structurally and functionally related lipid transfer proteins. This study aimed to investigate the associations of LBP with the lipid composition of human adipose tissue. METHODS: Lipidomic analysis was performed in whole adipose tissue. To validate the associations found, LBP was knocked down (KD) in 3T3-L1 adipocytes, and lipidomic profile was evaluated by nontargeted lipidomics. RESULTS:LBP gene expression was negatively associated with phosphatidylcholine, phosphatidylserine, and sphingomyelin relative abundance in vivo. LBP expression was also decreased in those samples with the highest docosahexanoic content, implicated in the resolution of inflammation. The KD of LBP (by ∼70%) led to sharp changes in the lipidome of adipocytes. Of note, specific plasmalogen species PE(O-16:0/22:5), PE(38:2), odd chain glycerolipids, and cholesteryl linoleate were upregulated by LBP KD. In contrast, GM3 gangliosides, several ceramides, a triacylglycerol potentially containing arachidonate, and cholesteryl palmitate were downregulated by LBP KD. CONCLUSIONS:LBP seems to play a role in the regulation of lipid composition of adipose tissue linked to resilience to inflammation.
Authors: Jessica Latorre; Ramon Díaz-Trelles; Ferran Comas; Aleix Gavaldà-Navarro; Edward Milbank; Nathalia Dragano; Samantha Morón-Ros; Rajesh Mukthavaram; Francisco Ortega; Anna Castells-Nobau; Núria Oliveras-Cañellas; Wifredo Ricart; Priya P Karmali; Kiyoshi Tachikawa; Pad Chivukula; Francesc Villarroya; Miguel López; Marta Giralt; José Manuel Fernández-Real; José María Moreno-Navarrete Journal: Mol Ther Nucleic Acids Date: 2022-08-05 Impact factor: 10.183