Luis P B Guerzoni1, Valérie Nicolas2, Angelina Angelova3. 1. Institut Galien Paris-Sud, CNRS UMR 8612, Univ. Paris-Sud, Université Paris-Saclay, LabEx LERMIT, 5 rue J.-B. Clément, 92296, Châtenay-Malabry cedex, France. 2. MIPSIT, Paris-Saclay Institute of Therapeutic Innovation (IPSIT-UMS3679 CNRS, US31 INSERM), Faculty of Pharmacy, Univ Paris Sud, Université Paris-Saclay, 5 rue J.-B. Clément, 92296, Châtenay-Malabry, France. 3. Institut Galien Paris-Sud, CNRS UMR 8612, Univ. Paris-Sud, Université Paris-Saclay, LabEx LERMIT, 5 rue J.-B. Clément, 92296, Châtenay-Malabry cedex, France. angelina.angelova@u-psud.fr.
Abstract
PURPOSE: To in vitro investigate the capacity of carrier-free and lipid-nanoparticle (NP)-encapsulated phytochemical compounds to prevent neuronal damage through neurotrophin potentiating activities. Delivery of molecules promoting the neurotrophin receptor signaling in the central nervous system (CNS) present ongoing interest for combination therapy development. METHODS: Super-resolution Stimulated Emission Depletion (STED) microscopy imaging and flow cytometry analysis were employed to study the expression of the neurotrophin TrkB receptor in a neuronal cell model, which is highly responsive to binding of brain-derived neurotrophic factor (BDNF). Dual drug-loaded nanoparticle formulations, prepared by self-assembly of lyotropic lipids and PEGylated amphiphile derivatives, were delivered to differentiated human neuroblastoma SH-SY5Y cells subjected to degenerative conditions. RESULTS: The expression of BDNF in the intra and extracellular domains was quantified by ELISA and flow cytometry after sequential treatment of the degenerating SH-SY5Y cells by neurotherapeutic formulations. Flow cytometry was also used to assess the phosphorylation of the transcription factor cAMP response element-binding protein (CREB) in the intracellular domain as a result of the treatment by nanoformulations. CONCLUSION: Over time, dual drug formulations (curcumin and docosahexaenoic acid (DHA)) promoted the neuronal survival and repair processes through enhanced BDNF secretion and increased phosphorylation of CREB as compared to untreated degenerating cells.
PURPOSE: To in vitro investigate the capacity of carrier-free and lipid-nanoparticle (NP)-encapsulated phytochemical compounds to prevent neuronal damage through neurotrophin potentiating activities. Delivery of molecules promoting the neurotrophin receptor signaling in the central nervous system (CNS) present ongoing interest for combination therapy development. METHODS: Super-resolution Stimulated Emission Depletion (STED) microscopy imaging and flow cytometry analysis were employed to study the expression of the neurotrophinTrkB receptor in a neuronal cell model, which is highly responsive to binding of brain-derived neurotrophic factor (BDNF). Dual drug-loaded nanoparticle formulations, prepared by self-assembly of lyotropic lipids and PEGylated amphiphile derivatives, were delivered to differentiated humanneuroblastoma SH-SY5Y cells subjected to degenerative conditions. RESULTS: The expression of BDNF in the intra and extracellular domains was quantified by ELISA and flow cytometry after sequential treatment of the degenerating SH-SY5Y cells by neurotherapeutic formulations. Flow cytometry was also used to assess the phosphorylation of the transcription factor cAMP response element-binding protein (CREB) in the intracellular domain as a result of the treatment by nanoformulations. CONCLUSION: Over time, dual drug formulations (curcumin and docosahexaenoic acid (DHA)) promoted the neuronal survival and repair processes through enhanced BDNF secretion and increased phosphorylation of CREB as compared to untreated degenerating cells.
Authors: Juliana B Hoppe; Karine Coradini; Rudimar L Frozza; Claudia M Oliveira; André B Meneghetti; Andressa Bernardi; Elisa Simões Pires; Ruy C R Beck; Christianne G Salbego Journal: Neurobiol Learn Mem Date: 2013-08-14 Impact factor: 2.877
Authors: Gamal M Zayed; Islam Kamal; Wael A Abdelhafez; Fahd M Alsharif; Mohamed A Amin; Montaser Sh A Shaykoon; Hatem A Sarhan; Ahmed M Abdelsalam Journal: Pharm Res Date: 2018-03-30 Impact factor: 4.200