Isolation, purification, structural analysis and immunostimulatory activity of water-soluble polysaccharides from Grifola Frondosa fruiting body.

A new polysaccharide had been successfully isolated from maitake mushroom (Grifola Frondosa)-GFP. HPLC and Monosaccharide analysis showed that the average molecular weight of GFP was 155kDa and it was mainly composed of rhamnose, xylose, mannose, glucose, molar ratio of 1.00: 1.04: 1.11: 6.21. FTIR, methylation analysis and NMR were used to analyze the structural characterization of GFP. Structural analysis results revealed that its backbone consisted of (1→4)-linked methylation, Glcp residues were major structural polysaccharide GFP units, accounting of the polysaccharide backbone speculate GFP every→3)-Glcp-(1→and one→3,4)-Glcp-(1→connected interval with a small amount of 1→, 1→4, 1→6 glycosidic linkage. MTT assay showed that GFP could significantly improve the proliferation activity of RAW264.7 cells in a certain range of concentrations and time. Scanningelectro microscopy (SEM) results indicated that GFP could induce RAW264.7 cells activation. GFP could obviously increase the proliferation index and enhance the immunostimulatory activity such as the cytokine and chemokine production.