Feng Shi1, Fang Xiao2, Ping Ding3, Hong Qin4, Ruixue Huang5. 1. Internal Medicine Department of Thyroid and Radionuclide Therapy, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China. 2. Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, China. 3. Center of Preventive Medicine Experiment, Xiangya School of Public Health, Central South University, Changsha, China. 4. Department of Nutrition and Food Hygiene, Xiangya School of Public Health, Central South University, China. 5. Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, China. Electronic address: huangruixue_ss@163.com.
Abstract
BACKGROUND AND AIMS: Long noncoding RNA (lncRNA) highly up-regulated in liver cancer (HULC) is the first identified lncRNA highly expressed in hepatocellular carcinoma and acts as an oncogenic lncRNA. However, the significance of HULC in triple-negative breast cancer (TNBC) is still unclear. Our purpose is to explore the clinical significance and biological function of HULC in TNBC. METHODS: Expression of HULC in TNBC tissues and cell lines was measured and the association between HULC expression and clinicopathological factors was analyzed. Cell proliferation, migration, and invasion assays were executed by using TNBC cell lines transfected with si-HULC or si-NC. The potential mechanism of HULC was explored by Western blot. RESULTS: HULC expression was increased in TNBC tissues and cell lines and associated with malignant status and poor prognosis of TNBC patients. Furthermore, silencing TNBC expression effectively suppressed TNBC cells metastasis through MMP-2 and MMP-9. CONCLUSION: HULC acts as an oncogene lncRNA in TNBC and as an independent poor prognostic factor in TNBC patients.
BACKGROUND AND AIMS: Long noncoding RNA (lncRNA) highly up-regulated in liver cancer (HULC) is the first identified lncRNA highly expressed in hepatocellular carcinoma and acts as an oncogenic lncRNA. However, the significance of HULC in triple-negative breast cancer (TNBC) is still unclear. Our purpose is to explore the clinical significance and biological function of HULC in TNBC. METHODS: Expression of HULC in TNBC tissues and cell lines was measured and the association between HULC expression and clinicopathological factors was analyzed. Cell proliferation, migration, and invasion assays were executed by using TNBC cell lines transfected with si-HULC or si-NC. The potential mechanism of HULC was explored by Western blot. RESULTS:HULC expression was increased in TNBC tissues and cell lines and associated with malignant status and poor prognosis of TNBC patients. Furthermore, silencing TNBC expression effectively suppressed TNBC cells metastasis through MMP-2 and MMP-9. CONCLUSION:HULC acts as an oncogene lncRNA in TNBC and as an independent poor prognostic factor in TNBC patients.