Literature DB >> 27982728

A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays.

Justina O Tam1, Helena de Puig2, Chun-Wan Yen1,3, Irene Bosch3, Jose Gómez-Márquez4, Charles Clavet1, Kimberly Hamad-Schifferli2,5, Lee Gehrke3,6.   

Abstract

Point-of-care (POC) diagnostics such as lateral flow and dipstick immunoassays use gold nanoparticle (NP)-antibody conjugates for visual readout. We investigated the effects of NP conjugation, surface chemistries, and antibody immobilization methods on dipstick performance. We compared orientational, covalent conjugation, electrostatic adsorption, and a commercial conjugation kit for dipstick assays to detect dengue virus NS1 protein. Assay performance depended significantly on their conjugate properties. We also tested arrangements of multiple test lines within strips. Results show that orientational, covalent conjugation with PEG shield could improve NS1 detection. These approaches can be used to optimize immunochromatographic detection for a range of biomarkers.

Entities:  

Keywords:  bioconjugation; dengue; dipstick; lateral flow; nanoparticle; rapid test

Mesh：

Substances：

Year:  2016        PMID： 27982728      PMCID： PMC6202116          DOI： 10.1080/15321819.2016.1269338

Source DB:  PubMed          Journal:  J Immunoassay Immunochem        ISSN： 1532-1819


  33 in total

1.  Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles.

Authors:  Konstantin Sokolov; Michele Follen; Jesse Aaron; Ina Pavlova; Anais Malpica; Reuben Lotan; Rebecca Richards-Kortum
Journal:  Cancer Res       Date:  2003-05-01       Impact factor: 12.701

2.  Gold nanoparticle-cytochrome C complexes: the effect of nanoparticle ligand charge on protein structure.

Authors:  Marie-Eve Aubin-Tam; Kimberly Hamad-Schifferli
Journal:  Langmuir       Date:  2005-12-20       Impact factor: 3.882

3.  Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties.

Authors:  S Kumar; J Aaron; K Sokolov
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

4.  Effect of architecture on the activity of glucose oxidase/horseradish peroxidase/carbon nanoparticle conjugates.

Authors:  Paula Ciaurriz; Ernesto Bravo; Kimberly Hamad-Schifferli
Journal:  J Colloid Interface Sci       Date:  2013-10-04       Impact factor: 8.128

5.  Paper based point-of-care testing disc for multiplex whole cell bacteria analysis.

Authors:  Chen-zhong Li; Katherine Vandenberg; Shradha Prabhulkar; Xuena Zhu; Lisa Schneper; Kalai Methee; Charles J Rosser; Eugenio Almeide
Journal:  Biosens Bioelectron       Date:  2011-04-28       Impact factor: 10.618

6.  Simple paper architecture modifications lead to enhanced sensitivity in nanoparticle based lateral flow immunoassays.

Authors:  Claudio Parolo; Mariana Medina-Sánchez; Alfredo de la Escosura-Muñiz; Arben Merkoçi
Journal:  Lab Chip       Date:  2013-02-07       Impact factor: 6.799

7.  High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever.

Authors:  Daniel H Libraty; Paul R Young; Darren Pickering; Timothy P Endy; Siripen Kalayanarooj; Sharone Green; David W Vaughn; Ananda Nisalak; Francis A Ennis; Alan L Rothman
Journal:  J Infect Dis       Date:  2002-09-16       Impact factor: 5.226

8.  Plasmon resonance coupling of metal nanoparticles for molecular imaging of carcinogenesis in vivo.

Authors:  Jesse Aaron; Nitin Nitin; Kort Travis; Sonia Kumar; Tom Collier; Sun Young Park; Miguel José-Yacamán; Lezlee Coghlan; Michele Follen; Rebecca Richards-Kortum; Konstantin Sokolov
Journal:  J Biomed Opt       Date:  2007 May-Jun       Impact factor: 3.170

9.  Determination of size and concentration of gold nanoparticles from UV-vis spectra.

Authors:  Wolfgang Haiss; Nguyen T K Thanh; Jenny Aveyard; David G Fernig
Journal:  Anal Chem       Date:  2007-04-26       Impact factor: 6.986

Review 10.  Nanobioimaging and sensing of infectious diseases.

Authors:  Padmavathy Tallury; Astha Malhotra; Logan M Byrne; Swadeshmukul Santra
Journal:  Adv Drug Deliv Rev       Date:  2009-11-25       Impact factor: 15.470
View more
  7 in total

1.  Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1.

Authors:  Sanjay Kumar; Pulak Bhushan; Vinay Krishna; Shantanu Bhattacharya
Journal:  Biomicrofluidics       Date:  2018-05-14       Impact factor: 2.800

Review 2.  Gold nanoparticles in virus detection: Recent advances and potential considerations for SARS-CoV-2 testing development.

Authors:  Jianxin Wang; Adam J Drelich; Caroline M Hopkins; Sandro Mecozzi; Lingjun Li; Glen Kwon; Seungpyo Hong
Journal:  Wiley Interdiscip Rev Nanomed Nanobiotechnol       Date:  2021-09-08

3.  Gold-based nanoplatform for a rapid lateral flow immunochromatographic test assay for gluten detection.

Authors:  Arefe Momeni; Mohammad Rostami-Nejad; Reza Salarian; Mohammad Rabiee; Elham Aghamohammadi; Mohammad Reza Zali; Navid Rabiee; Franklin R Tay; Pooyan Makvandi
Journal:  BMC Biomed Eng       Date:  2022-05-20

Review 4.  Challenges of the Nano-Bio Interface in Lateral Flow and Dipstick Immunoassays.

Authors:  Helena de Puig; Irene Bosch; Lee Gehrke; Kimberly Hamad-Schifferli
Journal:  Trends Biotechnol       Date:  2017-09-28       Impact factor: 19.536

5.  Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.

Authors:  Lusiani Dewi Assaat; Endang Saepudin; Retno Damayanti Soejoedono; Rahmat Setya Adji; Okti Nadia Poetri; Tribidasari Anggraningrum Ivandini
Journal:  J Adv Vet Anim Res       Date:  2019-08-06

Review 6.  The Impact of Engineered Silver Nanomaterials on the Immune System.

Authors:  Neethu Ninan; Nirmal Goswami; Krasimir Vasilev
Journal:  Nanomaterials (Basel)       Date:  2020-05-18       Impact factor: 5.719

7.  High-Sensitive Detection and Quantitative Analysis of Thyroid-Stimulating Hormone Using Gold-Nanoshell-Based Lateral Flow Immunoassay Device.

Authors:  Santosh Kumar Bikkarolla; Sara E McNamee; Paul Vance; James McLaughlin
Journal:  Biosensors (Basel)       Date:  2022-03-19
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.