| Literature DB >> 27978622 |
Yingying Meng1,2, Jing Zhang1,2, Fenglin Zhang1,2, Wei Ai1,2, Xiaotong Zhu1,2, Gang Shu1,2, Lina Wang1,2, Ping Gao1,2, Qianyun Xi1,2, Yongliang Zhang1,2, Xingwei Liang3, Qingyan Jiang1,2, Songbo Wang1,2.
Abstract
It has been demonstrated that dietary fat affects pubertal mammary gland development. However, the role of lauric acid (LA) in this process remains unclear. Thus, this study aimed to investigate the effects of LA on mammary gland development in pubertal mice and to explore the underlying mechanism. In vitro, 100 μM LA significantly promoted proliferation of mouse mammary epithelial cell line HC11 by regulating expression of proliferative markers (cyclin D1/3, p21, PCNA). Meanwhile, LA activated the G protein-coupled receptor 84 (GPR84) and PI3K/Akt signaling pathway. In agreement, dietary 1% LA enhanced mammary duct development, increased the expression of GPR84 and cyclin D1, and activated PI3K/Akt in mammary gland of pubertal mice. Furthermore, knockdown of GPR84 or inhibition of PI3K/Akt totally abolished the promotion of HC11 proliferation induced by LA. These results showed that LA stimulated mammary gland development of pubertal mice through activation of GPR84 and PI3K/Akt signaling pathway.Entities:
Keywords: GPR84; PI3K/Akt; lauric acid; mammary gland development; pubertal mice
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Year: 2016 PMID： 27978622 DOI： 10.1021/acs.jafc.6b04878
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279