Megan Ulmer Carnes1, Jane A Hoppin2, Nervana Metwali3, Annah B Wyss1, John L Hankinson4, Elizabeth Long O'Connell5, Marie Richards6, Stuart Long6, Laura E Beane Freeman7, Dale P Sandler1, Paul K Henneberger8, Christie Barker-Cummings5, David M Umbach9, Peter S Thorne3, Stephanie J London1. 1. 1 Epidemiology Branch and. 2. 2 Department of Biological Sciences, Center for Human Health and the Environment, North Carolina State University, Raleigh, North Carolina. 3. 3 Department of Occupational and Environmental Health, University of Iowa, Iowa City, Iowa. 4. 4 Hankinson Consulting, Inc., Athens, Georgia. 5. 5 Social & Scientific Systems, Inc., Durham, North Carolina. 6. 6 Westat, Durham, North Carolina. 7. 7 Occupational and Environmental Epidemiology Branch, National Cancer Institute, Bethesda, Maryland; and. 8. 8 Respiratory Health Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Department of Health and Human Services, Morgantown, West Virginia. 9. 9 Biostatistics and Computational Biology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina.
Abstract
RATIONALE: Endotoxin initiates a proinflammatory response from the innate immune system. Studies in children suggest that endotoxin exposure from house dust may be an important risk factor for asthma, but few studies have been conducted in adult populations. OBJECTIVES: To investigate the association of house dust endotoxin levels with asthma and related phenotypes (wheeze, atopy, and pulmonary function) in a large U.S. farming population. METHODS: Dust was collected from the bedrooms (n = 2,485) of participants enrolled in a case-control study of current asthma (927 cases) nested within the Agricultural Health Study. Dust endotoxin was measured by Limulus amebocyte lysate assay. Outcomes were measured by questionnaire, spirometry, and blood draw. We evaluated associations using linear and logistic regression. MEASUREMENTS AND MAIN RESULTS: Endotoxin was significantly associated with current asthma (odds ratio [OR], 1.30; 95% confidence interval [CI], 1.14-1.47), and this relationship was modified by early-life farm exposure (born on a farm: OR, 1.18; 95% CI, 1.02-1.37; not born on a farm: OR, 1.67; 95% CI, 1.26-2.20; Interaction P = 0.05). Significant positive associations were seen with both atopic and nonatopic asthma. Endotoxin was not related to either atopy or wheeze. Higher endotoxin was related to lower FEV1/FVC in asthma cases only (Interaction P = 0.01). For asthma, there was suggestive evidence of a gene-by-environment interaction for the CD14 variant rs2569190 (Interaction P = 0.16) but not for the TLR4 variants rs4986790 and rs4986791. CONCLUSIONS: House dust endotoxin was associated with current atopic and nonatopic asthma in a U.S. farming population. The degree of the association with asthma depended on early-life farm exposures. Furthermore, endotoxin was associated with lower pulmonary function in patients with asthma.
RATIONALE: Endotoxin initiates a proinflammatory response from the innate immune system. Studies in children suggest that endotoxin exposure from house dust may be an important risk factor for asthma, but few studies have been conducted in adult populations. OBJECTIVES: To investigate the association of house dust endotoxin levels with asthma and related phenotypes (wheeze, atopy, and pulmonary function) in a large U.S. farming population. METHODS: Dust was collected from the bedrooms (n = 2,485) of participants enrolled in a case-control study of current asthma (927 cases) nested within the Agricultural Health Study. Dust endotoxin was measured by Limulus amebocyte lysate assay. Outcomes were measured by questionnaire, spirometry, and blood draw. We evaluated associations using linear and logistic regression. MEASUREMENTS AND MAIN RESULTS: Endotoxin was significantly associated with current asthma (odds ratio [OR], 1.30; 95% confidence interval [CI], 1.14-1.47), and this relationship was modified by early-life farm exposure (born on a farm: OR, 1.18; 95% CI, 1.02-1.37; not born on a farm: OR, 1.67; 95% CI, 1.26-2.20; Interaction P = 0.05). Significant positive associations were seen with both atopic and nonatopic asthma. Endotoxin was not related to either atopy or wheeze. Higher endotoxin was related to lower FEV1/FVC in asthma cases only (Interaction P = 0.01). For asthma, there was suggestive evidence of a gene-by-environment interaction for the CD14 variant rs2569190 (Interaction P = 0.16) but not for the TLR4 variants rs4986790 and rs4986791. CONCLUSIONS: House dust endotoxin was associated with current atopic and nonatopic asthma in a U.S. farming population. The degree of the association with asthma depended on early-life farm exposures. Furthermore, endotoxin was associated with lower pulmonary function in patients with asthma.
Authors: Monika Werner; Rebekka Topp; Katrin Wimmer; Kai Richter; Wolfgang Bischof; Matthias Wjst; Joachim Heinrich Journal: J Allergy Clin Immunol Date: 2003-08 Impact factor: 10.793
Authors: Bart Ferwerda; Matthew Bb McCall; Karlijn Verheijen; Bart-Jan Kullberg; André Jam van der Ven; Jos Wm Van der Meer; Mihai G Netea Journal: Mol Med Date: 2008 May-Jun Impact factor: 6.354
Authors: Donna Carole Rennie; Joshua Allan Lawson; Ambikaipakan Senthilselvan; Philip James Willson; James Arnold Dosman Journal: Front Biosci (Elite Ed) Date: 2012-01-01
Authors: Christopher D Pascoe; Aruni Jha; Sujata Basu; Thomas Mahood; Amy Lee; Sam Hinshaw; Reza Falsafi; Robert E W Hancock; Neeloffer Mookherjee; Andrew J Halayko Journal: Am J Physiol Lung Cell Mol Physiol Date: 2020-04-22 Impact factor: 5.464
Authors: Brittney O Baumert; Megan Ulmer Carnes; Jane A Hoppin; Chandra L Jackson; Dale P Sandler; Laura Beane Freeman; Paul K Henneberger; David M Umbach; Srishti Shrestha; Stuart Long; Stephanie J London Journal: Sleep Health Date: 2017-09-28
Authors: Annah B Wyss; John S House; Jane A Hoppin; Marie Richards; John L Hankinson; Stuart Long; Paul K Henneberger; Laura E Beane Freeman; Dale P Sandler; Elizabeth Long O'Connell; Christie Barker Cummings; David M Umbach; Stephanie J London Journal: Thorax Date: 2017-07-08 Impact factor: 9.139
Authors: Kaitlyn G Lawrence; Nicole M Niehoff; Alexander P Keil; W Braxton Jackson; Kate Christenbury; Patricia A Stewart; Mark R Stenzel; Tran B Huynh; Caroline P Groth; Gurumurthy Ramachandran; Sudipto Banerjee; Gregory C Pratt; Matthew D Curry; Lawrence S Engel; Dale P Sandler Journal: Environ Int Date: 2022-07-27 Impact factor: 13.352
Authors: Robert M Burkes; Amanda J Gassett; Agathe S Ceppe; Wayne Anderson; Wanda K O'Neal; Prescott G Woodruff; Jerry A Krishnan; R Graham Barr; MeiLan K Han; Fernando J Martinez; Alejandro P Comellas; Allison A Lambert; Joel D Kaufman; Mark T Dransfield; J Michael Wells; Richard E Kanner; Robert Paine; Eugene R Bleecker; Laura M Paulin; Nadia N Hansel; M Bradley Drummond Journal: Ann Am Thorac Soc Date: 2018-07