| Literature DB >> 27975323 |
Jia Liu1,2, Xuan Huang2, Melanie Werner3, Ruth Broering3, Dongliang Yang1, Mengji Lu4.
Abstract
Separation of pure cell populations from the liver is a prerequisite to study the role of hepatic parenchymal and non-parenchymal cells in liver physiology, pathophysiology, and immunology. Traditional methods for hepatic cell separation usually purify only single cell types from liver specimens. Here, we describe an efficient method that can simultaneously purify populations of hepatocytes (HCs), liver sinusoidal endothelial cells (LSECs), and Kupffer cells (KCs) from a single mouse liver specimen. A liberase-based perfusion technique in combination with a low-speed centrifugation and magnetic-activated cell sorting (MACS) led to the isolation and purification of HCs, KCs, and LSECs with high yields and purity.Entities:
Keywords: Hepatocytes; Kupffer cells; Liver sinusoidal endothelial cells; Magnetic-activated cell sorting; Technology
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Year: 2017 PMID： 27975323 DOI： 10.1007/978-1-4939-6700-1_21
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745