Literature DB >> 27956860

Effect of Tween 80 and Acetone on the Secretion, 
Structure and Antioxidant Activities of Exopolysaccharides from Lentinus tigrinus.

Peixin He1, Shuangshuang Wu2, Lige Pan2, Siwen Sun2, Duobin Mao1, Chunping Xu1.   

Abstract

In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of Lentinus tigrinus exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS--C) were purified by Sepharose CL-6B gel filtration chromatography and molecular mass of purified fractions was estimated to be 22.1, 137 and 12 kDa, respectively. Monosaccharide composition analysis indicated that EPS-T, EPS-A and EPS-C were mainly composed of glucose and mannose. Congo Red test indicated that EPS-T and EPS-A had a highly ordered conformation of triple helix, while EPS-C had a random coil conformation. Furthermore, EPS-A exhibited higher DPPH scavenging and antiproliferative activities than EPS--C and EPS-T, which might be attributed to the molecular mass.

Entities:  

Keywords:  Lentinus tigrinus; antiproliferative activity; exopolysaccharides; molecular conformation; molecular mass

Year:  2016        PMID： 27956860      PMCID： PMC5151215          DOI： 10.17113/ftb.54.03.16.4211

Source DB:  PubMed          Journal:  Food Technol Biotechnol        ISSN： 1330-9862            Impact factor:   3.918


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