Literature DB >> 2794069

Rapid identification of low level hepatitis B-related viral genome in serum.

T J Liang1, K J Isselbacher, J R Wands.   

Abstract

A sensitive and specific method has been developed to detect hepatitis B virus (HBV) in serum. The method involves two steps: the capture of viral genome from serum using a high affinity IgM monoclonal antibody directed against a common a domain epitope found on the envelope, and the amplification of viral DNA by the polymerase chain reaction (PCR). The amplification is initiated using "generic" primers derived from the core and pre-core sequences which are highly conserved amongst the hepadnaviruses. This rapid technique detects less than 10 infectious virions and may be useful in the study of individuals with acute and chronic liver disease of unknown etiology.

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Year:  1989        PMID： 2794069      PMCID： PMC329801          DOI： 10.1172/JCI114308

Source DB:  PubMed          Journal:  J Clin Invest        ISSN： 0021-9738            Impact factor:   14.808


  18 in total

1.  Structural analysis of hepatitis B surface antigen by monoclonal antibodies.

Authors:  E Ben-Porath; J R Wands; R A Marciniak; M A Wong; L Hornstein; R Ryder; M Canlas; A Lingao; K J Isselbacher
Journal:  J Clin Invest       Date:  1985-10       Impact factor: 14.808

2.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

3.  Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

Authors:  U B Gyllensten; H A Erlich
Journal:  Proc Natl Acad Sci U S A       Date:  1988-10       Impact factor: 11.205

4.  Improved detection of hepatitis B surface antigen (HBsAg) in blood donors by monoclonal radioimmunoassay.

Authors:  E Ben-Porath; J R Wands; S Bar-Shany; C Huggins; K Isselbacher
Journal:  Transfusion       Date:  1985 Jan-Feb       Impact factor: 3.157

5.  Hepatitis B virus DNA in patients with chronic liver disease and negative tests for hepatitis B surface antigen.

Authors:  C Bréchot; F Degos; C Lugassy; V Thiers; S Zafrani; D Franco; H Bismuth; C Trépo; J P Benhamou; J Wands
Journal:  N Engl J Med       Date:  1985-01-31       Impact factor: 91.245

6.  Hepatitis B viral antigenic structure: signature analysis by monoclonal radioimmunoassays.

Authors:  J R Wands; M A Wong; J Shorey; R D Brown; R A Marciniak; K J Isselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

7.  Monoclonal radioimmunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes.

Authors:  D A Shafritz; H M Lieberman; K J Isselbacher; J R Wands
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

Review 8.  The hepatitis B virus.

Authors:  P Tiollais; C Pourcel; A Dejean
Journal:  Nature       Date:  1985 Oct 10-16       Impact factor: 49.962

9.  Common evolutionary origin of hepatitis B virus and retroviruses.

Authors:  R H Miller; W S Robinson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

10.  The complete nucleotide sequences of the cloned hepatitis B virus DNA; subtype adr and adw.

Authors:  Y Ono; H Onda; R Sasada; K Igarashi; Y Sugino; K Nishioka
Journal:  Nucleic Acids Res       Date:  1983-03-25       Impact factor: 16.971
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  11 in total

Review 1.  Hepatitis B virus. New and evolving issues.

Authors:  B Yoffe; C A Noonan
Journal:  Dig Dis Sci       Date:  1992-01       Impact factor: 3.199

2.  Quantitation of hepatitis B virus genomic DNA by real-time detection PCR.

Authors:  A Abe; K Inoue; T Tanaka; J Kato; N Kajiyama; R Kawaguchi; S Tanaka; M Yoshiba; M Kohara
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

3.  Frequent detection of hepatitis B virus X-gene DNA in hepatocellular carcinoma and adjacent liver tissue in hepatitis B surface antigen-negative patients.

Authors:  M Takeuchi; J Fujimoto; H Niwamoto; Y Yamamoto; E Okamoto
Journal:  Dig Dis Sci       Date:  1997-11       Impact factor: 3.199

4.  Acute hepatitis in rats expressing human hepatitis B virus transgenes.

Authors:  H Takahashi; J Fujimoto; S Hanada; K J Isselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  1995-02-28       Impact factor: 11.205

5.  Enhanced replication of a hepatitis B virus mutant associated with an epidemic of fulminant hepatitis.

Authors:  K Hasegawa; J Huang; S A Rogers; H E Blum; T J Liang
Journal:  J Virol       Date:  1994-03       Impact factor: 5.103

Review 6.  Polymerase chain reaction for the diagnosis of viral hepatitis B and C.

Authors:  C Bréchot
Journal:  Gut       Date:  1993       Impact factor: 23.059

7.  Solid-phase C1q-directed bacterial capture followed by PCR for detection of Chlamydia trachomatis in clinical specimens.

Authors:  P Herbrink; H A van den Munckhof; H G Niesters; W H Goessens; E Stolz; W G Quint
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

8.  Detection of pre-C and core region mutants of hepatitis B virus in chronic hepatitis B virus carriers.

Authors:  T Wakita; S Kakumu; M Shibata; K Yoshioka; Y Ito; T Shinagawa; T Ishikawa; M Takayanagi; T Morishima
Journal:  J Clin Invest       Date:  1991-12       Impact factor: 14.808

9.  Sensitivity of seven commercial assays in the detection of hepatitis B virus type 2-like infection.

Authors:  P León; J A López; J M Echevarría
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-07       Impact factor: 3.267

10.  Comparison of methods for detection of hepatitis B virus DNA.

Authors:  H L Zaaijer; F ter Borg; H T Cuypers; M C Hermus; P N Lelie
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948
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