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Literature DB >> 6182569

Monoclonal radioimmunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes.

D A Shafritz, H M Lieberman, K J Isselbacher, J R Wands.

Abstract

High-affinity monoclonal IgM and IgG antibodies (anti-HBs) to hepatitis B surface antigen (HBsAg) have recently been developed and used by us in the construction of highly sensitive radioimmunoassays for the detection of HBsAg-associated determinants in serum. We now report that selected serum samples demonstrating monoclonal immunoreactive material also contain nucleic acid sequences complementary to hepatitis B virus (HBV) DNA by molecular hybridization analysis. In addition, monoclonal radioimmunoassays can detect viral determinants in HBsAg-anti-HBs immune complexes formed in anti-HBs excess; such determinants are undetectable by commercial radioimmunoassay. These and our previous studies suggest that there are HBV or "HBV-related" agents present in human serum that are detected by monoclonal antibodies but are not identified by conventional polyvalent antibodies.

Entities: Disease Species

Mesh：

Substances：

Year: 1982 PMID： 6182569 PMCID： PMC346967 DOI： 10.1073/pnas.79.18.5675

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors: G Köhler; C Milstein
Journal: Nature Date: 1975-08-07 Impact factor: 49.962

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Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

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Authors: J R Wands; V R Zurawski
Journal: Gastroenterology Date: 1981-02 Impact factor: 22.682

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Authors: P R Chakraborty; N Ruiz-Opazo; D Shouval; D A Shafritz
Journal: Nature Date: 1980-07-31 Impact factor: 49.962

5. Comparison of serum hepatitis B surface antigen (HBsAg) and serum anticore with tissue HBsAg and hepatitis B core antigen (HBcAg).

Authors: M Omata; A Afroudakis; C T Liew; M Ashcavai; R L Peters
Journal: Gastroenterology Date: 1978-12 Impact factor: 22.682

6. Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.

Authors: J R Wands; R I Carlson; H Schoemaker; K J Isselbacher; V R Zurawski
Journal: Proc Natl Acad Sci U S A Date: 1981-02 Impact factor: 11.205

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Authors: J R Wands; E Mann; E Alpert; K J Isselbacher
Journal: J Clin Invest Date: 1975-05 Impact factor: 14.808

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Authors: J C Edman; P Gray; P Valenzuela; L B Rall; W J Rutter
Journal: Nature Date: 1980-07-31 Impact factor: 49.962

9. Hepatitis B core antigen in hepatocytes of patients with chronic active hepatitis.

Authors: C Brechot; C Trepo; D Ninova-Bradistilova; A Goudeau; C Degott; P Maupas; F Potet; J P Benhamou
Journal: Dig Dis Sci Date: 1980-08 Impact factor: 3.199

10. Chronic hepatitis in chimpanzee carriers of hepatitis B virus: morphologic, immunologic, and viral DNA studies.

Authors: D Shouval; P R Chakraborty; N Ruiz-Opazo; S Baum; I Spigland; E Muchmore; M A Gerber; S N Thung; H Popper; D A Shafritz
Journal: Proc Natl Acad Sci U S A Date: 1980-10 Impact factor: 11.205

15 in total

Review 1. Serological responses to HBV infection.

Authors: E A Fagan; R Williams
Journal: Gut Date: 1986-07 Impact factor: 23.059

2. Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization.

Authors: G H Keller; D P Huang; J W Shih; M M Manak
Journal: J Clin Microbiol Date: 1990-06 Impact factor: 5.948

3. Hepatitis B virus infection and primary liver cancer--an unfolding story.

Authors: D A Shafritz
Journal: West J Med Date: 1984-05

4. Detection and transmission in chimpanzees of hepatitis B virus-related agents formerly designated "non-A, non-B" hepatitis.

Authors: J R Wands; H M Lieberman; E Muchmore; K Isselbacher; D A Shafritz
Journal: Proc Natl Acad Sci U S A Date: 1982-12 Impact factor: 11.205

5. Detection of hepatitis B virus DNA in mononuclear blood cells.

Authors: P Pontisso; M C Poon; P Tiollais; C Brechot
Journal: Br Med J (Clin Res Ed) Date: 1984-05-26

6. Screening for hepatitis B virus in healthy blood donors by molecular DNA hybridization analysis.

Authors: C F Sun; C C Pao; S Y Wu; Y F Liaw
Journal: J Clin Microbiol Date: 1988-09 Impact factor: 5.948

7. Importance of showing HBsAg and HBcAg positivity in the liver for better aetiological definition of chronic liver disease.

Authors: L Cuccurullo; M Rambaldi; G Iaquinto; F Ferraraccio; L Ambrosone; N Giardullo; A De Vita
Journal: J Clin Pathol Date: 1987-02 Impact factor: 3.411