Raimundo Fernandes de Araújo1, Aurigena Antunes de Araújo2, Jonas Bispo Pessoa3, Franscisco Paulo Freire Neto4, Gisele Ribeiro da Silva2, Ana Luiza C S Leitão Oliveira5, Thaís Gomes de Carvalho5, Heloiza F O Silva6, Mateus Eugênio7, Celso Sant'Anna7, Luiz H S Gasparotto8. 1. Department of Morphology, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil; Post Graduation Programme in Structural and Functional Biology, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil; Post Graduation Programme in Health Science, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil. Electronic address: araujojr@cb.ufrn.br. 2. Department of Biophysics and Pharmacology, Post Graduation Programme in Public Health, Post graduation programme in Pharmaceutical Science, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil. 3. Post Graduation Programme in Structural and Functional Biology, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil. 4. Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil. 5. Post Graduation Programme in Health Science, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil. 6. Group of Biological Chemistry and Chemometrics, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil. 7. Laboratory of Biotechnology - Labio, National Institute of Metrology, Quality and Technology - Inmetro, Duque de Caxias 25250-020, RJ, Brazil. 8. Group of Biological Chemistry and Chemometrics, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil. Electronic address: lhgasparotto@ufrnet.br.
Abstract
BACKGROUND: Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. METHODS: GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV-vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV-vis and histopathological analysis, respectively. RESULTS: Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40μg/mL to 80μg/mL (p<0.05). The best anti-inflammatory activity was observed at the dose of 1500μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. CONCLUSION: The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems.
BACKGROUND: Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. METHODS: GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV-vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV-vis and histopathological analysis, respectively. RESULTS: Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40μg/mL to 80μg/mL (p<0.05). The best anti-inflammatory activity was observed at the dose of 1500μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. CONCLUSION: The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems.
Authors: Reza Shahbazi; Gabriella Sghia-Hughes; Jack L Reid; Sara Kubek; Kevin G Haworth; Olivier Humbert; Hans-Peter Kiem; Jennifer E Adair Journal: Nat Mater Date: 2019-05-27 Impact factor: 43.841