Ali Tekin1, Osman Zeki Karakuş2, Gülce Hakgüder2, Oğuz Ateş2, Erdener Özer3, Mustafa Olguner2, Feza Miraç Akgür2. 1. Departments of Pediatric Surgery, Section of Pediatric Urology, Ege University School of Medicine, İzmir, Turkey. 2. Departments of Pediatric Surgery, Dokuz Eylül University School of Medicine, İzmir, Turkey. 3. Departments of Pathology, Dokuz Eylül University School of Medicine, İzmir, Turkey.
Abstract
OBJECTIVE: Myelomeningocele (MMC) is one of the most common reason of neurogenic bladder dysfunction in children. Although neurogenic bladder dysfunction occurrence is related with bladder innervation, also there are some changes seen in the smooth muscle and neural cells of the bladder. Interstitial cells of Cajal (ICC) are the pacemaker cells found in organs with peristaltic activity. Although it has been shown that ICC are diminished in the rat urinary bladder with traumatic spinal cord injury, there is no data about ICC in fetal rat bladders with MMC. This study has been conducted to investigate the ICC in the bladders of fetal rats with retinoic acid induced MMC. MATERIALS AND METHODS: Time dated pregnant Wistar albino rats were divided into 3 groups. In MMC group, dams were fed with gavage solution containing 60 mg/kg all-trans retinoic acid dissolved in olive oil on 10. embryologic day. Sham group animals were fed only olive oil. Control group dams were fed with standard rat chow. Fetuses were delivered by cesarean section and harvested on 22. embryologic day. MMC was identified by observing MMC sacs at the back of the fetuses. Distribution of ICCs were evaluated using immunohistochemical staining. RESULTS: ICCs were found in all groups, which have the same morphological features that had been described earlier in the gastrointestinal tract and the bladder. The density of the ICC in the MMC group was found to be significantly decreased when compared with the control and the sham groups (p<0.05). CONCLUSION: The density of the ICC in the urinary bladder decreased in the neurogenic bladder developed in MMC.
OBJECTIVE: Myelomeningocele (MMC) is one of the most common reason of neurogenic bladder dysfunction in children. Although neurogenic bladder dysfunction occurrence is related with bladder innervation, also there are some changes seen in the smooth muscle and neural cells of the bladder. Interstitial cells of Cajal (ICC) are the pacemaker cells found in organs with peristaltic activity. Although it has been shown that ICC are diminished in the rat urinary bladder with traumatic spinal cord injury, there is no data about ICC in fetal rat bladders with MMC. This study has been conducted to investigate the ICC in the bladders of fetal rats with retinoic acid induced MMC. MATERIALS AND METHODS: Time dated pregnant Wistar albino rats were divided into 3 groups. In MMC group, dams were fed with gavage solution containing 60 mg/kg all-transretinoic acid dissolved in olive oil on 10. embryologic day. Sham group animals were fed only olive oil. Control group dams were fed with standard rat chow. Fetuses were delivered by cesarean section and harvested on 22. embryologic day. MMC was identified by observing MMC sacs at the back of the fetuses. Distribution of ICCs were evaluated using immunohistochemical staining. RESULTS: ICCs were found in all groups, which have the same morphological features that had been described earlier in the gastrointestinal tract and the bladder. The density of the ICC in the MMC group was found to be significantly decreased when compared with the control and the sham groups (p<0.05). CONCLUSION: The density of the ICC in the urinary bladder decreased in the neurogenic bladder developed in MMC.
Authors: Louise Johnston; Rebecca M J Cunningham; John S Young; Christopher H Fry; Gordon McMurray; Rachel Eccles; Karen D McCloskey Journal: J Cell Mol Med Date: 2012-07 Impact factor: 5.310
Authors: Minoru Miyazato; Katsumi Kadekawa; Takeya Kitta; Naoki Wada; Nobutaka Shimizu; William C de Groat; Lori A Birder; Anthony J Kanai; Seiichi Saito; Naoki Yoshimura Journal: Urol Clin North Am Date: 2017-08 Impact factor: 2.241