Ahmed Amara1,2, Meriem Mrad1,2, Aicha Sayeh1,2, Dhaker Lahideb3,4, Samy Layouni1,5, Abdeddayem Haggui3,4, Najiba Fekih-Mrissa1,6, Habib Haouala3,4, Brahim Nsiri1,5. 1. 1 Laboratoire de Biologie Moléculaire, Service d'Hématologie, Hôpital Militaire de Tunis, Montfleury, Tunisie. 2. 2 Faculté des Sciences de Tunis, Université Tunis el Manar, Tunis, Tunisie. 3. 3 Service de Cardiologie, Hôpital Militaire de Tunis, Montfleury, Tunisie. 4. 4 Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis, Tunisie. 5. 5 Faculté de Pharmacie, Université de Monastir, Monastir, Tunisie. 6. 6 Académie Militaire Fondouk Jédid, Nabeul, Tunisie.
Abstract
BACKGROUND: Coronary artery disease (CAD), also known as atherosclerotic heart disease, is a leading cause of mortality and morbidity throughout the world. The role of insertion/deletion (I/D) polymorphisms of the angiotensin-converting enzyme (ACE) gene in the etiology of CAD remains to be more completely clarified. The aim of this study was to determine the role of the ACE I/D polymorphism in patients with CAD and to study the association together with traditional risk factors in assessing the risk of CAD. METHODS: Our study population included 145 Tunisian patients with symptomatic CAD and a control group of 300 people matched for age and sex. All participants in the study were genotyped for the ACE I/D polymorphisms obtained by polymerase chain reaction amplification on genomic DNA. RESULTS: Our analysis showed that the ACE D allele frequency ( P < 10-3; odds ratio [OR] = 5.2; 95% confidence interval [CI] = 3.6-7.6) and DD genotype ( P < 10-3; OR = 6.8; 95% CI = 4.4-10) are significantly more prevalent among patients with CAD than in controls and may be predisposing to CAD. We further found that the risk of CAD is greatly potentiated by several concomitant risk factors (smoking, diabetes, hypertension, dyslipidemia, and a family history of CAD). CONCLUSION: The ACE D allele may be predictive in individuals who may be at risk of developing CAD. Further investigations of these polymorphisms and their possible synergisms with traditional risk factors for CAD could help to ascertain better predictability for CAD susceptibility.
BACKGROUND:Coronary artery disease (CAD), also known as atherosclerotic heart disease, is a leading cause of mortality and morbidity throughout the world. The role of insertion/deletion (I/D) polymorphisms of the angiotensin-converting enzyme (ACE) gene in the etiology of CAD remains to be more completely clarified. The aim of this study was to determine the role of the ACE I/D polymorphism in patients with CAD and to study the association together with traditional risk factors in assessing the risk of CAD. METHODS: Our study population included 145 Tunisian patients with symptomatic CAD and a control group of 300 people matched for age and sex. All participants in the study were genotyped for the ACE I/D polymorphisms obtained by polymerase chain reaction amplification on genomic DNA. RESULTS: Our analysis showed that the ACE D allele frequency ( P < 10-3; odds ratio [OR] = 5.2; 95% confidence interval [CI] = 3.6-7.6) and DD genotype ( P < 10-3; OR = 6.8; 95% CI = 4.4-10) are significantly more prevalent among patients with CAD than in controls and may be predisposing to CAD. We further found that the risk of CAD is greatly potentiated by several concomitant risk factors (smoking, diabetes, hypertension, dyslipidemia, and a family history of CAD). CONCLUSION: The ACE D allele may be predictive in individuals who may be at risk of developing CAD. Further investigations of these polymorphisms and their possible synergisms with traditional risk factors for CAD could help to ascertain better predictability for CAD susceptibility.
Entities:
Keywords:
ACE gene polymorphism; coronary artery disease; traditional risk factors
Authors: Aadil Yousif; Rashid Mir; Jamsheed Javid; Jameel Barnawi; Mohammed M Jalal; Malik A Altayar; Salem Owaid Albalawi; Faisel M Abuduhier Journal: Diagnostics (Basel) Date: 2022-05-26
Authors: Denise S Pinheiro; Rodrigo S Santos; Paulo C B Veiga Jardim; Elisangela G Silva; Angela A S Reis; Gustavo R Pedrino; Cirano J Ulhoa Journal: PLoS One Date: 2019-08-20 Impact factor: 3.240