Literature DB >> 2789513

Deletion of 3' untranslated region of human BSF-2 mRNA causes stabilization of the mRNA and high-level expression in mouse NIH3T3 cells.

N Tonouchi1, K Miwa, H Karasuyama, H Matsui.   

Abstract

Most lymphokine genes have an mRNA-destabilizing signal in their 3' untranslated sequences. In this study we demonstrated the usefulness of deletion of this sequence for lymphokine production. By expression of B cell stimulatory factor 2 (BSF-2) cDNAs with and without this region in mouse NIH3T3 cells, it was shown that mRNA of BSF-2 with the 3' destabilizing sequence was very unstable, and that by deletion of this region, the mRNA was stabilized. More than 10-fold BSF-2 productivity was observed in cells harboring the plasmid without the 3' region than in those with it.

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Year:  1989        PMID： 2789513     DOI： 10.1016/0006-291x(89)92328-0

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

1.  Autoregulatory effect of interleukin-10 on proinflammatory cytokine production by Porphyromonas gingivalis lipopolysaccharide-tolerant human monocytes.

Authors:  H Shimauchi; T Ogawa; K Okuda; Y Kusumoto; H Okada
Journal:  Infect Immun       Date:  1999-05       Impact factor: 3.441

2.  Characterization of a family of genes encoding a fruit-specific wound-stimulated protein of bell pepper (Capsicum annuum): identification of a new family of transposable elements.

Authors:  J Pozueta-Romero; M Klein; G Houlné; M L Schantz; B Meyer; R Schantz
Journal:  Plant Mol Biol       Date:  1995-09       Impact factor: 4.076

3.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

4.  An examination of the generation-time effect on molecular evolution.

Authors:  T Ohta
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-15       Impact factor: 11.205

5.  Evolutionary conservation of the AU-rich 3' untranslated region of messenger RNA.

Authors:  M A Asson-Batres; S L Spurgeon; J Diaz; T G DeLoughery; G C Bagby
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-15       Impact factor: 11.205

6.  Regulation of cytokine production in the human thymus: epidermal growth factor and transforming growth factor alpha regulate mRNA levels of interleukin 1 alpha (IL-1 alpha), IL-1 beta, and IL-6 in human thymic epithelial cells at a post-transcriptional level.

Authors:  P T Le; S Lazorick; L P Whichard; B F Haynes; K H Singer
Journal:  J Exp Med       Date:  1991-11-01       Impact factor: 14.307
  6 in total

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