AIM: Differences in children's development and susceptibility to diseases and exposures have been observed by sex, yet human studies of sex differences in miRNAs are limited. MATERIALS & METHODS: The genome-wide miRNA expression was characterized by sequencing-based EdgeSeq assay in cord blood buffy coats from 89 newborns, and 564 miRNAs were further analyzed. RESULTS: Differential expression of most miRNAs was higher in boys. Neurodevelopment, RNA metabolism and metabolic ontology terms were enriched among miRNA targets. The majority of upregulated miRNAs (86%) validated by nCounter maintained positive-fold change values; however, only 21% reached statistical significance by false discovery rate. CONCLUSION: Accounting for host factors like sex may improve the sensitivity of epigenetic analyses for epidemiological studies in early childhood.
AIM: Differences in children's development and susceptibility to diseases and exposures have been observed by sex, yet human studies of sex differences in miRNAs are limited. MATERIALS & METHODS: The genome-wide miRNA expression was characterized by sequencing-based EdgeSeq assay in cord blood buffy coats from 89 newborns, and 564 miRNAs were further analyzed. RESULTS: Differential expression of most miRNAs was higher in boys. Neurodevelopment, RNA metabolism and metabolic ontology terms were enriched among miRNA targets. The majority of upregulated miRNAs (86%) validated by nCounter maintained positive-fold change values; however, only 21% reached statistical significance by false discovery rate. CONCLUSION: Accounting for host factors like sex may improve the sensitivity of epigenetic analyses for epidemiological studies in early childhood.
Entities:
Keywords:
cord blood; early life; epigenetics; filtering; miRNA; miRNAome; newborns; next-generation sequencing; normalization procedures; sex differences
Authors: D M Morales-Prieto; W Chaiwangyen; S Ospina-Prieto; U Schneider; J Herrmann; B Gruhn; U R Markert Journal: Placenta Date: 2012-06-19 Impact factor: 3.481
Authors: Julia E Rager; Kathryn A Bailey; Lisa Smeester; Sloane K Miller; Joel S Parker; Jessica E Laine; Zuzana Drobná; Jenna Currier; Christelle Douillet; Andrew F Olshan; Marisela Rubio-Andrade; Miroslav Stýblo; Gonzalo García-Vargas; Rebecca C Fry Journal: Environ Mol Mutagen Date: 2013-12-10 Impact factor: 3.216
Authors: Markus Sällman Almén; Emil K Nilsson; Josefin A Jacobsson; Ineta Kalnina; Janis Klovins; Robert Fredriksson; Helgi B Schiöth Journal: Gene Date: 2014-07-08 Impact factor: 3.688
Authors: Emmett E Whitaker; Brianne Z Wiemann; Jason C Xia; Bruno Bissonnette; Joseph Liu; Paolo Fadda; Joseph D Tobias; Fievos L Christofi Journal: J Anesth Date: 2019-10-14 Impact factor: 2.078
Authors: Nicole Ludwig; Anne Hecksteden; Mustafa Kahraman; Tobias Fehlmann; Thomas Laufer; Fabian Kern; Tim Meyer; Eckart Meese; Andreas Keller; Christina Backes Journal: RNA Biol Date: 2019-05-10 Impact factor: 4.652
Authors: Eiichiro Satake; Marcus G Pezzolesi; Zaipul I Md Dom; Adam M Smiles; Monika A Niewczas; Andrzej S Krolewski Journal: Diabetes Date: 2018-02-16 Impact factor: 9.461
Authors: Gary P Brennan; Dimitrios M Vitsios; Sophie Casey; Ann-Marie Looney; Boubou Hallberg; David C Henshall; Geraldine B Boylan; Deirdre M Murray; Catherine Mooney Journal: PLoS One Date: 2018-12-03 Impact factor: 3.240
Authors: Karen Huen; Daneida Lizarraga; Katherine Kogut; Brenda Eskenazi; Nina Holland Journal: Int J Environ Res Public Health Date: 2019-02-13 Impact factor: 3.390
Authors: Allison Kupsco; Diddier Prada; Damaskini Valvi; Lisa Hu; Maria Skaalum Petersen; Brent Coull; Philippe Grandjean; Pal Weihe; Andrea A Baccarelli Journal: Sci Rep Date: 2021-03-12 Impact factor: 4.379
Authors: Maria Tsamou; Karen Vrijens; Congrong Wang; Ellen Winckelmans; Kristof Y Neven; Narjes Madhloum; Theo M de Kok; Tim S Nawrot Journal: Epigenetics Date: 2020-09-06 Impact factor: 4.528