Maria Vetro1, Dodi Safari2, Silvia Fallarini3, Korrie Salsabila4, Martina Lahmann5, Soledad Penadés6, Luigi Lay7, Marco Marradi6,8, Federica Compostella9. 1. Present address: Center for Synthesis & Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland. 2. Eijkman Institute for Molecular Biology, Jakarta, Indonesia. 3. Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale, Largo Donegani 2, 28100 Novara, Italy. 4. Faculty of Biology, Jenderal Soedirman University, Purwokerto, Indonesia. 5. Bangor University, Bangor, Gwynedd, LL57 2UW, Wales, UK. 6. Laboratory of GlycoNanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE & CIBER-BBN, Paseo Miramón 182, 20009, San Sebastián, Spain. 7. Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy. 8. Present address: IK4-CIDETEC, Paseo Miramón 196, 20009, San Sebastián, Spain. 9. Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli Studi di Milano, Via Saldini 50, 20133 Milano, Italy.
Abstract
AIM: Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two synthetic carbohydrate antigens related to serotypes 19F and 14 of Streptococcus pneumoniae and evaluated their immunogenicity in vivo. RESULTS: A tetrasaccharide fragment of serotype 14 (Tetra-14), a trisaccharide fragment of serotype 19F (Tri-19F), a T-helper peptide and d -glucose were loaded onto GNP in different ratios. Mice immunization showed that the concomitant presence of Tri-19F and Tetra-14 on the same nanoparticle critically enhanced the titers of specific IgG antibodies toward type 14 polysaccharide compared with GNP exclusively displaying Tetra-14, while no IgG antibodies against type 19F polysaccharide were elicited. CONCLUSION: This work is a step forward toward synthetic nanosystems combining carbohydrate antigens and immunogenic peptides as potential carbohydrate-based vaccines.
AIM: Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two synthetic carbohydrate antigens related to serotypes 19F and 14 of Streptococcus pneumoniae and evaluated their immunogenicity in vivo. RESULTS: A tetrasaccharide fragment of serotype 14 (Tetra-14), a trisaccharide fragment of serotype 19F (Tri-19F), a T-helper peptide and d -glucose were loaded onto GNP in different ratios. Mice immunization showed that the concomitant presence of Tri-19F and Tetra-14 on the same nanoparticle critically enhanced the titers of specific IgG antibodies toward type 14 polysaccharide compared with GNP exclusively displaying Tetra-14, while no IgG antibodies against type 19F polysaccharide were elicited. CONCLUSION: This work is a step forward toward synthetic nanosystems combining carbohydrate antigens and immunogenic peptides as potential carbohydrate-based vaccines.
Authors: Hector Terán-Navarro; Ricardo Calderon-Gonzalez; David Salcines-Cuevas; Isabel García; Marco Marradi; Javier Freire; Erwan Salmon; Mar Portillo-Gonzalez; Elisabet Frande-Cabanes; Almudena García-Castaño; Virginia Martinez-Callejo; Javier Gomez-Roman; Raquel Tobes; Fernando Rivera; Sonsoles Yañez-Diaz; Carmen Álvarez-Domínguez Journal: Oncoimmunology Date: 2018-11-10 Impact factor: 8.110
Authors: Marko Anderluh; Francesco Berti; Anna Bzducha-Wróbel; Fabrizio Chiodo; Cinzia Colombo; Federica Compostella; Katarzyna Durlik; Xhenti Ferhati; Rikard Holmdahl; Dragana Jovanovic; Wieslaw Kaca; Luigi Lay; Milena Marinovic-Cincovic; Marco Marradi; Musa Ozil; Laura Polito; Josè Juan Reina; Celso A Reis; Robert Sackstein; Alba Silipo; Urban Švajger; Ondřej Vaněk; Fumiichiro Yamamoto; Barbara Richichi; Sandra J van Vliet Journal: FEBS J Date: 2021-06-01 Impact factor: 5.622