Literature DB >> 27864512

Epstein-Barr virus super-enhancer eRNAs are essential for MYC oncogene expression and lymphoblast proliferation.

Jun Liang1,2, Hufeng Zhou1,2, Catherine Gerdt1, Min Tan1, Tyler Colson1, Kenneth M Kaye1, Elliott Kieff3,2, Bo Zhao3.   

Abstract

Epstein-Barr virus (EBV) super-enhancers (ESEs) are essential for lymphoblastoid cell (LCL) growth and survival. Reanalyses of LCL global run-on sequencing (Gro-seq) data found abundant enhancer RNAs (eRNAs) being transcribed at ESEs. Inactivation of ESE components, EBV nuclear antigen 2 (EBNA2) and bromodomain-containing protein 4 (BRD4), significantly decreased eRNAs at ESEs -428 and -525 kb upstream of the MYC oncogene transcription start site (TSS). shRNA knockdown of the MYC -428 and -525 ESE eRNA caused LCL growth arrest and reduced cell growth. Furthermore, MYC ESE eRNA knockdown also significantly reduced MYC expression, ESE H3K27ac signals, and MYC ESEs looping to MYC TSS. These data indicate that ESE eRNAs strongly affect cell gene expression and enable LCL growth.

Entities:  

Keywords:  Epstein–Barr virus; MYC; eRNA; super-enhancer

Mesh：

Substances：

Year:  2016        PMID： 27864512      PMCID： PMC5150416          DOI： 10.1073/pnas.1616697113

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  46 in total

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Journal:  Science       Date:  2014-11-13       Impact factor: 47.728

2.  Epstein-Barr virus latent membrane protein 1 is essential for B-lymphocyte growth transformation.

Authors:  K M Kaye; K M Izumi; E Kieff
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205

3.  The Epstein-Barr virus nuclear protein 2 acidic domain can interact with TFIIB, TAF40, and RPA70 but not with TATA-binding protein.

Authors:  X Tong; F Wang; C J Thut; E Kieff
Journal:  J Virol       Date:  1995-01       Impact factor: 5.103

4.  Analysis of nascent RNA identifies a unified architecture of initiation regions at mammalian promoters and enhancers.

Authors:  Leighton J Core; André L Martins; Charles G Danko; Colin T Waters; Adam Siepel; John T Lis
Journal:  Nat Genet       Date:  2014-11-10       Impact factor: 38.330

5.  Genetic analysis of immortalizing functions of Epstein-Barr virus in human B lymphocytes.

Authors:  W Hammerschmidt; B Sugden
Journal:  Nature       Date:  1989-08-03       Impact factor: 49.962

6.  Epstein-Barr virus nuclear antigen 2 transactivates latent membrane protein LMP1.

Authors:  F Wang; S F Tsang; M G Kurilla; J I Cohen; E Kieff
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

7.  Epigenetic repression of p16(INK4A) by latent Epstein-Barr virus requires the interaction of EBNA3A and EBNA3C with CtBP.

Authors:  Lenka Skalska; Robert E White; Melanie Franz; Michaela Ruhmann; Martin J Allday
Journal:  PLoS Pathog       Date:  2010-06-10       Impact factor: 6.823

8.  The EBNA-2 arginine-glycine domain is critical but not essential for B-lymphocyte growth transformation; the rest of region 3 lacks essential interactive domains.

Authors:  X Tong; R Yalamanchili; S Harada; E Kieff
Journal:  J Virol       Date:  1994-10       Impact factor: 5.103

9.  Brd4 activates P-TEFb for RNA polymerase II CTD phosphorylation.

Authors:  Friederike Itzen; Ann Katrin Greifenberg; Christian A Bösken; Matthias Geyer
Journal:  Nucleic Acids Res       Date:  2014-05-23       Impact factor: 16.971

10.  Integrator mediates the biogenesis of enhancer RNAs.

Authors:  Fan Lai; Alessandro Gardini; Anda Zhang; Ramin Shiekhattar
Journal:  Nature       Date:  2015-08-26       Impact factor: 49.962
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  42 in total

1.  Super-enhancers for RUNX3 are required for cell proliferation in EBV-infected B cell lines.

Authors:  Hiroki Hosoi; Akiko Niibori-Nambu; Giselle Sek Suan Nah; Avinash Govind Bahirvani; Michelle Meng Huang Mok; Takaomi Sanda; Alan Prem Kumar; Daniel G Tenen; Yoshiaki Ito; Takashi Sonoki; Motomi Osato
Journal:  Gene       Date:  2021-01-12       Impact factor: 3.688

2.  TAF Family Proteins and MEF2C Are Essential for Epstein-Barr Virus Super-Enhancer Activity.

Authors:  Chong Wang; Sizun Jiang; Luyao Zhang; Difei Li; Jun Liang; Yohei Narita; Isabella Hou; Qian Zhong; Benjamin E Gewurz; Mingxiang Teng; Bo Zhao
Journal:  J Virol       Date:  2019-07-30       Impact factor: 5.103

3.  Enhancer RNAs in cancer: regulation, mechanisms and therapeutic potential.

Authors:  Joo-Hyung Lee; Feng Xiong; Wenbo Li
Journal:  RNA Biol       Date:  2020-01-19       Impact factor: 4.652

Review 4.  The structural and functional roles of CTCF in the regulation of cell type-specific and human disease-associated super-enhancers.

Authors:  Ha Youn Shin
Journal:  Genes Genomics       Date:  2018-11-19       Impact factor: 1.839

5.  EBV latent membrane protein 2A orchestrates p27kip1 degradation via Cks1 to accelerate MYC-driven lymphoma in mice.

Authors:  Kamonwan Fish; Richard P Sora; Samantha J Schaller; Richard Longnecker; Masato Ikeda
Journal:  Blood       Date:  2017-10-26       Impact factor: 22.113

Review 6.  Current Progress in EBV-Associated B-Cell Lymphomas.

Authors:  Yonggang Pei; Alexandria E Lewis; Erle S Robertson
Journal:  Adv Exp Med Biol       Date:  2017       Impact factor: 2.622

Review 7.  Nanog Expression in Embryonic Stem Cells - An Ideal Model System to Dissect Enhancer Function.

Authors:  Steven Blinka; Sridhar Rao
Journal:  Bioessays       Date:  2017-10-04       Impact factor: 4.345

8.  Global epigenomic analysis of KSHV-infected primary effusion lymphoma identifies functional MYC superenhancers and enhancer RNAs.

Authors:  Angela Park; Soohwan Oh; Kyle L Jung; Un Yung Choi; Hye-Ra Lee; Michael G Rosenfeld; Jae U Jung
Journal:  Proc Natl Acad Sci U S A       Date:  2020-08-18       Impact factor: 11.205

9.  The Epstein-Barr Virus Regulome in Lymphoblastoid Cells.

Authors:  Sizun Jiang; Hufeng Zhou; Jun Liang; Catherine Gerdt; Chong Wang; Liangru Ke; Stefanie C S Schmidt; Yohei Narita; Yijie Ma; Shuangqi Wang; Tyler Colson; Benjamin Gewurz; Guoliang Li; Elliott Kieff; Bo Zhao
Journal:  Cell Host Microbe       Date:  2017-10-11       Impact factor: 21.023

Review 10.  Epigenetic crossroads of the Epstein-Barr virus B-cell relationship.

Authors:  Thomas C Frost; Benjamin E Gewurz
Journal:  Curr Opin Virol       Date:  2018-09-15       Impact factor: 7.090
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