Nasimudeen R Jabir1, Chelapram K Firoz1, Mohammad A Kamal1, Ghazi A Damanhouri1, Mohammed Nabil Alama2, Anas S Alzahrani3, Hussein A Almehdar4, Shams Tabrez1. 1. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia. 2. Department of Cardiology, Faculty of Medicine, King Abdulaziz University Hospital, Jeddah, Saudi Arabia. 3. Department of Internal Medicine, Faculty of Medicine, University of Jeddah, Jeddah, Saudi Arabia. 4. Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Abstract
BACKGROUND: The present study consisted of a total of 200 subjects (100 confirmed coronary artery disease (CAD) patients), both men and women, and 100 healthy control individuals. METHODS: Serum concentration of IL-6 and RANTES were measured by enzyme-linked immunosorbent assay kit. For SNPs analysis, sanger method of DNA sequencing was followed. RESULTS: We observed variable numbers of SNP sites at -174 G/C, -572 G/C, and -597 G/A in IL-6 and -28 C/G and -109 C/T in RANTES promoters in CAD patients compared with control individuals. However, the observed changes in the number of SNPs were found to be non-significant compared with control individuals. The IL-6 level was found to be significantly (P<.001) elevated in CAD patients compared with control. Moreover, RANTES serum level did not show any significant change in CAD patients. CONCLUSION: Based on our result, it is quite clear that inflammation has a role in the pathogenesis of CAD but does not lead to significant changes at the genetic level in our population. As far as our knowledge goes, this is the first report that shows the genetic diversity in IL-6 and RANTES promoters and their respective levels in Saudi CAD patients.
BACKGROUND: The present study consisted of a total of 200 subjects (100 confirmed coronary artery disease (CAD) patients), both men and women, and 100 healthy control individuals. METHODS: Serum concentration of IL-6 and RANTES were measured by enzyme-linked immunosorbent assay kit. For SNPs analysis, sanger method of DNA sequencing was followed. RESULTS: We observed variable numbers of SNP sites at -174 G/C, -572 G/C, and -597 G/A in IL-6 and -28 C/G and -109 C/T in RANTES promoters in CAD patients compared with control individuals. However, the observed changes in the number of SNPs were found to be non-significant compared with control individuals. The IL-6 level was found to be significantly (P<.001) elevated in CAD patients compared with control. Moreover, RANTES serum level did not show any significant change in CAD patients. CONCLUSION: Based on our result, it is quite clear that inflammation has a role in the pathogenesis of CAD but does not lead to significant changes at the genetic level in our population. As far as our knowledge goes, this is the first report that shows the genetic diversity in IL-6 and RANTES promoters and their respective levels in Saudi CAD patients.
Authors: Donald M Lloyd-Jones; Peter W F Wilson; Martin G Larson; Alexa Beiser; Eric P Leip; Ralph B D'Agostino; Daniel Levy Journal: Am J Cardiol Date: 2004-07-01 Impact factor: 2.778
Authors: G Ed Rainger; Myriam Chimen; Matthew J Harrison; Clara M Yates; Paul Harrison; Stephen P Watson; Marie Lordkipanidzé; Gerard B Nash Journal: Platelets Date: 2015-07-21 Impact factor: 3.862