Sergey Shaposhnikov1,2, Thomas Hatzold3,4, Naouale El Yamani5, Philip Mark Stavro3,6, Yolanda Lorenzo7,5, Maria Dusinska8, Astrid Reus9, Wilrike Pasman10, Andrew Collins7,5,11. 1. NorGenoTech AS, Totenvegen 2049, 2848, Skreia, Norway. sas@norgenotech.no. 2. Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316, Oslo, Norway. sas@norgenotech.no. 3. Kraft Foods R & D Inc., Zweigniederlassung München, PB 830550, 81705, Munich, Germany. 4. , Johann-Straub-Weg 8, 81927, Muenchen, Germany. 5. Department of Nutrition, Faculty of Medicine, University of Oslo, PB 1046 Blindern, 0316, Oslo, Norway. 6. Bunge North America, St. Louis, MO, USA. 7. NorGenoTech AS, Totenvegen 2049, 2848, Skreia, Norway. 8. Department of Environmental Chemistry, Norwegian Institute for Air Research, PB 100, 2027, Kjeller, Norway. 9. TNO Triskelion, Zeist, The Netherlands. 10. TNO, Zeist, The Netherlands. 11. Comet BioTech AS, Instituttveien 18, 2007, Kjeller, Norway.
Abstract
PURPOSE: Coffee is known to contain phytochemicals with antioxidant potential. The aim of this study was to investigate possible antioxidant effects of coffee in healthy human volunteers. METHODS: A placebo-controlled intervention trial was carried out on 160 healthy human subjects, randomised into three groups, receiving 3 or 5 cups of study coffee or water per day, for 8 weeks. Blood samples were taken before, during, and after the intervention. Serum was used for analysis of blood lipids and standard clinical chemistry analytes. Peripheral blood mononuclear cells were isolated, and DNA damage (strand breaks and oxidised bases) was measured with the comet assay. The lipid oxidation product isoprostane 8-iso-PGF2α was assayed in urine samples by LC-MS/MS. RESULTS: There was no significant effect of coffee consumption on the markers of oxidation of DNA and lipids. Creatinine (in serum) increased by a few per cent in all groups, and the liver enzyme γ-glutamyl transaminase was significantly elevated in serum in the 5 cups/day group. Other clinical markers (including glucose and insulin), cholesterol, triacylglycerides, and inflammatory markers were unchanged. There was no effect of coffee on blood pressure. CONCLUSION: In a carefully controlled clinical trial with healthy subjects, up to 5 cups of coffee per day had no detectable effect, either beneficial or harmful, on human health.
RCT Entities:
PURPOSE: Coffee is known to contain phytochemicals with antioxidant potential. The aim of this study was to investigate possible antioxidant effects of coffee in healthy human volunteers. METHODS: A placebo-controlled intervention trial was carried out on 160 healthy human subjects, randomised into three groups, receiving 3 or 5 cups of study coffee or water per day, for 8 weeks. Blood samples were taken before, during, and after the intervention. Serum was used for analysis of blood lipids and standard clinical chemistry analytes. Peripheral blood mononuclear cells were isolated, and DNA damage (strand breaks and oxidised bases) was measured with the comet assay. The lipid oxidation product isoprostane 8-iso-PGF2α was assayed in urine samples by LC-MS/MS. RESULTS: There was no significant effect of coffee consumption on the markers of oxidation of DNA and lipids. Creatinine (in serum) increased by a few per cent in all groups, and the liver enzyme γ-glutamyl transaminase was significantly elevated in serum in the 5 cups/day group. Other clinical markers (including glucose and insulin), cholesterol, triacylglycerides, and inflammatory markers were unchanged. There was no effect of coffee on blood pressure. CONCLUSION: In a carefully controlled clinical trial with healthy subjects, up to 5 cups of coffee per day had no detectable effect, either beneficial or harmful, on human health.
Entities:
Keywords:
Coffee; DNA oxidation; Human intervention trial; Lipid oxidation
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