| Literature DB >> 27844169 |
Hua Li1, Bao Shi Wang1, You Ran Li1, Liang Zhang1, Zhong Yang Ding1, Zheng Hua Gu1, Gui Yang Shi2.
Abstract
In this study, we constructed an L-methionine-producing recombinant strain from wild-type Escherichia coli W3110 by metabolic engineering. To enhance the carbon flux to methionine and derepression met regulon, thrBC, lysA, and metJ were deleted in turn. Methionine biosynthesis obstacles were overcome by overexpression of metA Fbr (Fbr, Feedback resistance), metB, and malY under control of promoter pN25. Recombinant strain growth and methionine production were further improved by attenuation of metK gene expression through replacing native promoter by metK84p. Blocking the threonine pathway by deletion of thrBC or thrC was compared. Deletion of thrC showed faster growth rate and higher methionine production. Finally, metE, metF, and metH were overexpressed to enhance methylation efficiency. Compared with the original strain E. coli W3110, the finally obtained Me05 (pETMAFbr-B-Y/pKKmetH) improved methionine production from 0 to 0.65 and 5.62 g/L in a flask and a 15-L fermenter, respectively.Entities:
Keywords: Biosynthesis obstacle; Escherichia coli; L-homoserine; L-methionine; Metabolic engineering
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Year: 2016 PMID： 27844169 DOI： 10.1007/s10295-016-1870-3
Source DB: PubMed Journal: J Ind Microbiol Biotechnol ISSN： 1367-5435 Impact factor: 3.346