Natalia Karolina Kordulewska1, Elżbieta Kostyra2, Anna Cieślińska3, Michał Matysiewicz4, Ewa Fiedorowicz5, Edyta Sienkiewicz-Szłapka6. 1. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: natalia.smulska@uwm.edu.pl. 2. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: elzbieta.kostyra@uwm.edu.pl. 3. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: anna.cieslinska@uwm.edu.pl. 4. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: michal.matysiewicz@uwm.edu.pl. 5. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: ewa.kuzbida@uwm.edu.pl. 6. University of Warmia and Mazury in Olsztyn, Olsztyn, Warmińsko-Mazurskie, Poland. Electronic address: edyta.sienkiewicz@uwm.edu.pl.
Abstract
INTRODUCTION: Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-κB-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1β and CCL5/RANTES in incubated peripheral blood mononuclear cells (PBMC) to compared how histamine, FXF and osthole had influence on expression level and interacts on product secretion. OBJECTIVE: The purpose was to investigate expression pattern in asthma PBMC. METHODS: The cultures were treated 72h with FXF and osthole. We measured mRNA expression of histamine HRH-1, COX-2, NF-κB-p50, CCR1, IL-1β and CCL5/RANTES with Real-Time PCR (RT-PCR). RESULTS: The present study suggest that osthole may be a potential inhibitor of histamine H1 receptor activity. We also demonstrated that cells cultured with histamine increase COX-2 mRNA expression and osthole reduce it. CONCLUSION: Allergy remains one of the most common chronic diseases in Europe and it is rapidly approaching epidemic proportions; with current predictions estimating that the number of allergy-afflicted will equal the healthy population by 2020. It is therefore paramount to find new pharmaceuticals which successfully combat allergic disease.
INTRODUCTION:Fexofenadine (FXF) is a third-generation antihistamine drug and osthole is assumed as a natural antihistamine alternative. This paper compares results of histamine, FXF and osthole impact on HRH-1, COX-2, NF-κB-p50, CCR1 mRNA expression. We also measured mRNA expression of IL-1β and CCL5/RANTES in incubated peripheral blood mononuclear cells (PBMC) to compared how histamine, FXF and osthole had influence on expression level and interacts on product secretion. OBJECTIVE: The purpose was to investigate expression pattern in asthma PBMC. METHODS: The cultures were treated 72h with FXF and osthole. We measured mRNA expression of histamineHRH-1, COX-2, NF-κB-p50, CCR1, IL-1β and CCL5/RANTES with Real-Time PCR (RT-PCR). RESULTS: The present study suggest that osthole may be a potential inhibitor of histamine H1 receptor activity. We also demonstrated that cells cultured with histamine increase COX-2 mRNA expression and osthole reduce it. CONCLUSION:Allergy remains one of the most common chronic diseases in Europe and it is rapidly approaching epidemic proportions; with current predictions estimating that the number of allergy-afflicted will equal the healthy population by 2020. It is therefore paramount to find new pharmaceuticals which successfully combat allergic disease.
Authors: Tao Zhu; Xue Zhang; Xiaoting Chen; Anthony P Brown; Matthew T Weirauch; Theresa W Guilbert; Gurjit K Khurana Hershey; Jocelyn M Biagini; Hong Ji Journal: Allergy Date: 2020-11-25 Impact factor: 13.146
Authors: Soon Young Shin; Da Hyun Lee; Jishin Lee; Chan Choi; Ji-Young Kim; Jeong-Seok Nam; Yoongho Lim; Young Han Lee Journal: Oncotarget Date: 2017-10-10