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Literature DB >> 2783921

Isolation and characterization of a mutant of Haemophilus influenzae type b deficient in outer membrane protein P1.

Abstract

The gene for outer membrane protein P1 of Haemophilus influenzae type b has been previously cloned and expressed in Escherichia coli. To investigate the physiologic role of the P1 protein, the cloned P1 gene was insertionally inactivated with the Tn5 derivative Tn5tac1, and an isogenic P1-deficient Haemophilus mutant was then generated by transformation with linearized plasmid DNA containing the insertionally inactivated gene. The P1-deficient strain grew normally in vitro and induced bacteremia in the infant rat model.

Entities: Disease Species

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Year: 1989 PMID： 2783921 PMCID： PMC313214 DOI： 10.1128/iai.57.3.1002-1004.1989

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

12 in total

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Journal: Antimicrob Agents Chemother Date: 2010-12-28 Impact factor: 5.191

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Journal: Infect Immun Date: 1989-11 Impact factor: 3.441

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Authors: Amna Saeed-Kothe; Wei Yang; Scott D Mills
Journal: Appl Environ Microbiol Date: 2004-07 Impact factor: 4.792

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Authors: Marina Harper; John D Boyce; Ian W Wilkie; Ben Adler
Journal: Infect Immun Date: 2003-09 Impact factor: 3.441

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Journal: Infect Immun Date: 1992-04 Impact factor: 3.441