| Literature DB >> 27837488 |
Kyaw Aung1,2, Xiufang Xin1, Christy Mecey1, Sheng Yang He3,4,5.
Abstract
Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector systems are a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors that target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular basis of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.Entities:
Keywords: Agrobacterium; Arabidopsis thaliana; Bacterial pathogenesis; Confocal microscopy; Plant immunity; Plant pathogen; Tobacco; Type III secretion
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Year: 2017 PMID： 27837488 PMCID： PMC5643156 DOI： 10.1007/978-1-4939-6649-3_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745