Yuzhen Wei1,2, Jianjing Sun3, Xingang Li4,5. 1. Department of Neurosurgery, Qilu Hospital of Shandong University, 107 of Wenhua Xi Road Lixia District, Jinan, 250012, Shandong, People's Republic of China. 2. Department of Neurosurgery, Jining No.1 People's Hospital, Jining, Shandong, People's Republic of China. 3. Department of Endocrinology, Jining No.1 People's Hospital, Jining, 272 011, Shandong, People's Republic of China. 4. Department of Neurosurgery, Qilu Hospital of Shandong University, 107 of Wenhua Xi Road Lixia District, Jinan, 250012, Shandong, People's Republic of China. lixingangmed@yeah.net. 5. Brain Science Research Institute, Shandong University, Jinan, Shandong, People's Republic of China. lixingangmed@yeah.net.
Abstract
OBJECTIVE: To elucidate the molecular mechanism of microRNA-215 (miR-215) in the migration and invasion of high grade glioma. RESULTS: 42 Patients were analysed for clinicopathological characteristics. qRT-PCR showed that miR-215 was up-regulated in glioma tissues compared with non-neoplastic brain tissues (P < 0.05). The up-regulated miR-215 was closely associated with high grade glioma (P < 0.01) and poor overall survival (P < 0.01). Transwell assay showed that re-expression of miR-215 enhanced migration and invasion of glioma cells. miR-215 also down-regulated retinoblastoma tumor suppressor gene 1 (RB1) expression by targeting its 3'-UTR. Reversely, re-expression of RB1 inhibited partial effect of miR-215 on migration and invasion in vitro. CONCLUSIONS: Re-expression of miR-215 promoted cell migration and invasion of glioma by targeting RB1. miR-215 can thus be used as a biomarker for tumor progression and prognosis in human high grade glioma.
OBJECTIVE: To elucidate the molecular mechanism of microRNA-215 (miR-215) in the migration and invasion of high grade glioma. RESULTS: 42 Patients were analysed for clinicopathological characteristics. qRT-PCR showed that miR-215 was up-regulated in glioma tissues compared with non-neoplastic brain tissues (P < 0.05). The up-regulated miR-215 was closely associated with high grade glioma (P < 0.01) and poor overall survival (P < 0.01). Transwell assay showed that re-expression of miR-215 enhanced migration and invasion of glioma cells. miR-215 also down-regulated retinoblastoma tumor suppressor gene 1 (RB1) expression by targeting its 3'-UTR. Reversely, re-expression of RB1 inhibited partial effect of miR-215 on migration and invasion in vitro. CONCLUSIONS: Re-expression of miR-215 promoted cell migration and invasion of glioma by targeting RB1. miR-215 can thus be used as a biomarker for tumor progression and prognosis in human high grade glioma.
Authors: T Machackova; P Vychytilova-Faltejskova; K Souckova; R Laga; L Androvič; G Mixová; O Slaby Journal: Physiol Res Date: 2021-05-12 Impact factor: 1.881