| Literature DB >> 27832591 |
Danyang Gong1, Yong Hoon Kim1, Yuchen Xiao2, Yushen Du1, Yafang Xie1, Kevin K Lee3, Jun Feng1, Nisar Farhat1, Dawei Zhao1, Sara Shu1, Xinghong Dai1, Sumit K Chanda4, Tariq M Rana5, Nevan J Krogan6, Ren Sun1, Ting-Ting Wu7.
Abstract
Nuclear mRNA export is highly regulated to ensure accurate cellular gene expression. Viral inhibition of cellular mRNA export can enhance viral access to the cellular translation machinery and prevent anti-viral protein production but is generally thought to be nonselective. We report that ORF10 of Kaposi's sarcoma-associated herpesvirus (KSHV), a nuclear DNA virus, inhibits mRNA export in a transcript-selective manner to control cellular gene expression. Nuclear export inhibition by ORF10 requires an interaction with an RNA export factor, Rae1. Genome-wide analysis reveals a subset of cellular mRNAs whose nuclear export is blocked by ORF10 with the 3' UTRs of ORF10-targeted transcripts conferring sensitivity to export inhibition. The ORF10-Rae1 interaction is important for the virus to express viral genes and produce infectious virions. These results suggest that a nuclear DNA virus can selectively interfere with RNA export to restrict host gene expression for optimal replication. Published by Elsevier Inc.Entities:
Keywords: 3′ UTR; KSHV; Nup98; ORF10; Rae1; herpesvirus; late gene; mRNA nuclear export
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Year: 2016 PMID： 27832591 PMCID： PMC5130111 DOI： 10.1016/j.chom.2016.10.004
Source DB: PubMed Journal: Cell Host Microbe ISSN： 1931-3128 Impact factor: 21.023