Mónica Molano1,2,3, Pablo Moreno-Acosta4,2, Nicolás Morales1, Marcela Burgos1, Lina Buitrago5, Oscar Gamboa2,5, Rayner Alvarez1,2, Suzanne M Garland3,6,7, Sepehr N Tabrizi3,6,7, Renske D M Steenbergen8, Juan Carlos Mejía9. 1. Research Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia. 2. Research Group in Radiobiology Clinical, Molecular and Cellular, National Cancer Institute, Bogotá, Colombia. 3. Microbiology and Infection Diseases, The Royal Women´s Hospital, Melbourne, VIC, Australia. 4. Research Group in Cancer Biology, Research Branch, National Cancer Institute, Bogotá, Colombia pmoreno@cancer.gov.co. 5. Unit Group of Analysis, Research Branch, National Cancer Institute, Bogotá, Colombia. 6. Murdoch Children's Research Institute, Parkville, VIC, Australia. 7. Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australia. 8. Department of Pathology, VU University Medical Center, Amsterdam, the Netherlands. 9. Oncological Pathology Group, National Cancer Institute, Bogotá, Colombia.
Abstract
BACKGROUND: There exists limited information on the role of hTERT methylation, and its association with type-specific HPV infections in cervical cancer. MATERIALS AND METHODS: Eighty-seven frozen samples were analyzed for type-specific HPV infection using a GP5+/GP6+ PCR-RLB assay (RLB). hTERT DNA methylation analysis was performed using a newly developed PCR-RLB-hTERT. RESULTS: Ninety-three percent of samples were HPV-positive and fifteen different types were detected. hTERT methylation analysis of region 1 revealed no methylation in 78.8% of the samples and partial methylation in 21.2%. In region two, 68.2% showed no methylation and 31.8% showed a pattern of partial methylation. An association between the alpha 9 and alpha 7 species with a pattern of no methylation of hTERT in the region 1 was established (p=0.02 and p=0.03, respectively). CONCLUSION: Differences in patterns of methylation of the hTERT core promoter [region 1 (nt -208 to -1) and region 2 (nt +1 to +104) relative to first ATG] are related to the HPV species present. Copyright
BACKGROUND: There exists limited information on the role of hTERT methylation, and its association with type-specific HPV infections in cervical cancer. MATERIALS AND METHODS: Eighty-seven frozen samples were analyzed for type-specific HPV infection using a GP5+/GP6+ PCR-RLB assay (RLB). hTERT DNA methylation analysis was performed using a newly developed PCR-RLB-hTERT. RESULTS: Ninety-three percent of samples were HPV-positive and fifteen different types were detected. hTERT methylation analysis of region 1 revealed no methylation in 78.8% of the samples and partial methylation in 21.2%. In region two, 68.2% showed no methylation and 31.8% showed a pattern of partial methylation. An association between the alpha 9 and alpha 7 species with a pattern of no methylation of hTERT in the region 1 was established (p=0.02 and p=0.03, respectively). CONCLUSION: Differences in patterns of methylation of the hTERT core promoter [region 1 (nt -208 to -1) and region 2 (nt +1 to +104) relative to first ATG] are related to the HPV species present. Copyright
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