Silvia Pastoriza de la Cueva1, Juana Álvarez1, Ákos Végvári2, Javier Montilla-Gómez1, Olga Cruz-López3, Cristina Delgado-Andrade4, José A Rufián-Henares5. 1. Departamento de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Granada, Granada, Spain. 2. Clinical Protein Science & Imaging, Department of Biomedical Engineering, Lund University, Lund, Sweden. 3. Departamento de Química Farmacéutica y Orgánica, Facultad de Farmacia, Universidad de Granada, Granada, Spain. 4. Departamento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidin (EEZ-CSIC), Granada, Spain. 5. Departamento de Nutrición y Bromatología, Instituto de Investigación Biosanitaria (IBS), GRANADA, Universidad de Granada, Granada, Spain.
Abstract
SCOPE: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. METHODS AND RESULTS: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. CONCLUSION: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.
SCOPE: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. METHODS AND RESULTS: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. CONCLUSION: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.
Authors: Anna Magiera; Monika Ewa Czerwińska; Aleksandra Owczarek; Anna Marchelak; Sebastian Granica; Monika Anna Olszewska Journal: Molecules Date: 2022-05-20 Impact factor: 4.927
Authors: Silvia Pastoriza de la Cueva; Isabel Seiquer; Marta Mesías; José Ángel Rufián-Henares; Cristina Delgado-Andrade Journal: Foods Date: 2017-01-11
Authors: Mayada R Farag; Mahmoud Alagawany; May Bin-Jumah; Sarah I Othman; Asmaa F Khafaga; Hazem M Shaheen; Dalia Samak; Abdelrazeq M Shehata; Ahmed A Allam; Mohamed E Abd El-Hack Journal: Molecules Date: 2020-04-22 Impact factor: 4.411