| Literature DB >> 27799422 |
Yong Cui1,2,3,4, Qiong Zhao1,2,3,4, Hong-Tao Xie1,2,3,4, Wing Shing Wong1,2,3,4, Xiangfeng Wang1,2,3,4, Caiji Gao1,2,3,4, Yu Ding1,2,3,4, Yuqi Tan1,2,3,4, Takashi Ueda1,2,3,4, Yan Zhang1,2,3,4, Liwen Jiang5,6,7,8.
Abstract
Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating tapetal PCD Here, we demonstrate that Rab7-mediated vacuolar transport of tapetum degradation-related cysteine proteases is crucial for tapetal PCD and pollen development in Arabidopsis (Arabidopsis thaliana), with the following evidence: (1) The monensin sensitivity1 (mon1) mutants, which are defective in Rab7 activation, showed impaired male fertility due to a combined defect in both tapetum and male gametophyte development. (2) In anthers, MON1 showed preferential high level expression in tapetal cell layers and pollen. (3) The mon1 mutants exhibited delayed tapetum degeneration and tapetal PCD, resulting in abnormal pollen coat formation and decreased male fertility. (4) MON1/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-mediated Rab7 activation was indispensable for vacuolar trafficking of tapetum degradation-related cysteine proteases, supporting that PCD-triggered tapetum degeneration requires Rab7-mediated vacuolar trafficking of these cysteine proteases. (5) MON1 mutations also resulted in defective pollen germination and tube growth. Taken together, tapetal PCD and pollen development require successful MON1/CCZ1-mediated vacuolar transport in Arabidopsis.Entities:
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Year: 2016 PMID： 27799422 PMCID： PMC5210713 DOI： 10.1104/pp.16.00988
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340