| Literature DB >> 27797501 |
Jacob H Forstater1,2, Kyle Briggs3, Joseph W F Robertson1, Jessica Ettedgui1,2, Olivier Marie-Rose4, Canute Vaz1, John J Kasianowicz1, Vincent Tabard-Cossa3, Arvind Balijepalli1.
Abstract
Biological and solid-state nanometer-scale pores are the basis for numerous emerging analytical technologies for use in precision medicine. We developed Modular Single-Molecule Analysis Interface (MOSAIC), an open source analysis software that improves the accuracy and throughput of nanopore-based measurements. Two key algorithms are implemented: ADEPT, which uses a physical model of the nanopore system to characterize short-lived events that do not reach their steady-state current, and CUSUM+, a version of the cumulative sum statistical method optimized for longer events that do. We show that ADEPT detects previously unreported conductance states that occur as double-stranded DNA translocates through a 2.4 nm solid-state nanopore and reveals new interactions between short single-stranded DNA and the vestibule of a biological pore. These findings demonstrate the utility of MOSAIC and the ADEPT algorithm, and offer a new tool that can improve the analysis of nanopore-based measurements.Entities:
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Year: 2016 PMID： 27797501 PMCID： PMC5516951 DOI： 10.1021/acs.analchem.6b03725
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986