Literature DB >> 27797078

A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.

Jinbo Fan1, Hui Jin1, Hong-Guo Yu2.   

Abstract

In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a cis-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis.

Entities:  

Keywords:  Cohesin; Fluorescence-based reporter assay; Green fluorescence protein; Meiosis; Red fluorescence protein; Ty1-LTR

Mesh：

Substances：

Year:  2017        PMID： 27797078      PMCID： PMC5551346          DOI： 10.1007/978-1-4939-6545-8_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  LacZ assays in yeast.

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Authors:  Adele L Marston; Wai-Hong Tham; Hiral Shah; Angelika Amon
Journal:  Science       Date:  2004-01-29       Impact factor: 47.728

3.  High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method.

Authors:  R Daniel Gietz; Robert H Schiestl
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4.  Role of Polo-like kinase CDC5 in programming meiosis I chromosome segregation.

Authors:  Brian H Lee; Angelika Amon
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5.  Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8p.

Authors:  R S Cha; B M Weiner; S Keeney; J Dekker; N Kleckner
Journal:  Genes Dev       Date:  2000-02-15       Impact factor: 11.361

6.  Remodeling yeast gene transcription by activating the Ty1 long terminal repeat retrotransposon under severe adenine deficiency.

Authors:  Géraldine Servant; Carole Pennetier; Pascale Lesage
Journal:  Mol Cell Biol       Date:  2008-06-30       Impact factor: 4.272

7.  Comprehensive genetic analysis of transcription factor pathways using a dual reporter gene system in budding yeast.

Authors:  Pinay Kainth; Holly Elizabeth Sassi; Lourdes Peña-Castillo; Gordon Chua; Timothy R Hughes; Brenda Andrews
Journal:  Methods       Date:  2009-03-06       Impact factor: 3.608

8.  Scc2 regulates gene expression by recruiting cohesin to the chromosome as a transcriptional activator during yeast meiosis.

Authors:  Weiqiang Lin; Hui Jin; Xiuwen Liu; Kristin Hampton; Hong-Guo Yu
Journal:  Mol Biol Cell       Date:  2011-04-20       Impact factor: 4.138

9.  Transcriptional dysregulation in NIPBL and cohesin mutant human cells.

Authors:  Jinglan Liu; Zhe Zhang; Masashige Bando; Takehiko Itoh; Matthew A Deardorff; Dinah Clark; Maninder Kaur; Stephany Tandy; Tatsuro Kondoh; Eric Rappaport; Nancy B Spinner; Hugo Vega; Laird G Jackson; Katsuhiko Shirahige; Ian D Krantz
Journal:  PLoS Biol       Date:  2009-05-26       Impact factor: 8.029

10.  NIPBL, encoding a homolog of fungal Scc2-type sister chromatid cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange syndrome.

Authors:  Emma T Tonkin; Tzu-Jou Wang; Steven Lisgo; Michael J Bamshad; Tom Strachan
Journal:  Nat Genet       Date:  2004-05-16       Impact factor: 38.330
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  1 in total

1.  The half-bridge component Kar1 promotes centrosome separation and duplication during budding yeast meiosis.

Authors:  Meenakshi Agarwal; Hui Jin; Melainia McClain; Jinbo Fan; Bailey A Koch; Sue L Jaspersen; Hong-Guo Yu
Journal:  Mol Biol Cell       Date:  2018-05-30       Impact factor: 4.138
  1 in total

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