Draft Whole-Genome Sequence of a Haemophilus quentini Strain Isolated from an Infant in the United Kingdom.

Haemophilus quentini is a rare and distinct genospecies of Haemophilus that has been suggested as a cause of neonatal bacteremia and urinary tract infections in men. We present the draft whole-genome sequence of H. quentini MP1 isolated from an infant in the United Kingdom, aiding future identification and detection of this pathogen.

GENOME ANNOUNCEMENT

A distinct genospecies of nontypeable Haemophilus that is clinically relevant has recently been described which falls into the group Haemophilus influenzae biotype IV, with the name “Haemophilus quentini” having been proposed for the group (1–6). Haemophilus quentini has been found in the male urogenital tract and has been suggested as the cause of urinary tract infections in men, albeit very rarely (1, 3). Haemophilus quentini has also been recently identified as the cause of bacteremia in neonates in Italy and China (2, 4), in both cases it was isolated and identified through 16s rRNA gene sequencing (2, 4).

An unknown culture was isolated from an infant in the United Kingdom and was identified to be Haemophilus haemolyticus through sequencing of the outer membrane protein following amplification with primers targeting the P6 outer membrane protein of Haemophilus species and comparing the sequence to those available in public databases. However, it was also identified as H. quentini through sequencing of the 16s rRNA gene, similar to how it was previously identified in Italy and China (2, 4). The lack of homology of the P6 outer membrane gene product was therefore found to be due to there not being a published H. quentini genome.

Whole-genomic DNA was extracted from H. quentini MP1 using a combination of QiaSymphony DSP DNA minikit (Qiagen, United Kingdom) and High Pure viral nucleic acid kit (Roche, United Kingdom). A library was prepared with Nextera DNA library preparation kit (Illumina), and sequenced with Illumina MiSeq (150-cycle Reagent Kit v3), which generated ~1.36 million 2 × 76 bp paired-end reads. Raw data quality was evaluated using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc), and fastq-mcf (https://expressionanalysis.github.io/ea-utils/) was used to remove adaptors and low-quality sequences (

It is hoped that by publishing this draft genome it will aid the identification and detection of H. quentini in clinical samples in the future. Further analysis of the genome, including comprehensive characterization, reference guided assembly, and comparison to both H. haemolyticus and H. influenzae published genomes is currently in progress to identify any distinct regions and genes.

Accession number(s).

This whole-genome shotgun project has been deposited at GenBank under the accession no. MCII00000000. The version described in this paper is version MCII01000000.