Literature DB >> 26059717

BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.

Felipe A Simão1, Robert M Waterhouse1, Panagiotis Ioannidis1, Evgenia V Kriventseva1, Evgeny M Zdobnov1.   

Abstract

MOTIVATION: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.
RESULTS: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.
AVAILABILITY AND IMPLEMENTATION: Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT: evgeny.zdobnov@unige.ch SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
© The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Mesh:

Year:  2015        PMID: 26059717     DOI: 10.1093/bioinformatics/btv351

Source DB:  PubMed          Journal:  Bioinformatics        ISSN: 1367-4803            Impact factor:   6.937


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