Literature DB >> 2779373

Identification of cholesta-7,24-dien-3 beta-ol and desmosterol in hamster cauda epididymal spermatozoa.

M Awano1, A Kawaguchi, M Morisaki, H Mohri.   

Abstract

The sterol composition of hamster cauda epididymal spermatozoa was remarkably different from that of several other mammalian spermatozoa. Desmosterol and cholesta-7,24-dien-3 beta-ol account for as much as 90% of the total sterols. Cholesterol and desmosterol are the major components of mouse cauda epididymal spermatozoa, and rabbit, boar and bull ejaculated spermatozoa. Cholesta-7,24-dien-3 beta-ol was not detected. Furthermore, cholesterol was the main sterol in hamster caput epididymal spermatozoa, while only a trace amount of desmosterol was detected and cholesta-7,24-dien-3 beta-ol was hardly detected at all. The sterol content of cauda and caput epididymal spermatozoa was 0.17 +/- 0.05 mumol/10(8) spermatozoa. During maturation, the desmosterol and cholesta-7,24-dien-3 beta-ol levels increase and the cholesterol level decreases. Cholesta-7,24-dien-3 beta-ol appears as a sterol in mature spermatozoa and seems to be a characteristic sterol of hamster cauda epididymal spermatozoa.

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Year:  1989        PMID: 2779373     DOI: 10.1007/BF02535086

Source DB:  PubMed          Journal:  Lipids        ISSN: 0024-4201            Impact factor:   1.880


  9 in total

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Journal:  Exp Cell Res       Date:  1980-05       Impact factor: 3.905

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Authors:  N Ogihara; M Morisaki
Journal:  Chem Pharm Bull (Tokyo)       Date:  1988-07       Impact factor: 1.645

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Authors:  B K Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

5.  Changes in the lipid content of boar sperm plasma membranes during epididymal maturation.

Authors:  M Nikolopoulou; D A Soucek; J C Vary
Journal:  Biochim Biophys Acta       Date:  1985-05-28

6.  Interaction of lipids with the plasma membrane of sperm cells. I. The antifertilization action of cholesterol.

Authors:  B K Davis
Journal:  Arch Androl       Date:  1980-11

7.  Development changes occurring in the lipids of ram epididymal spermatozoa plasma membrane.

Authors:  J E Parks; R H Hammerstedt
Journal:  Biol Reprod       Date:  1985-04       Impact factor: 4.285

8.  Characterization of nystatin-resistant mutants of Saccharomyces cerevisiae and preparation of sterol intermediates using the mutants.

Authors:  S Nakanishi; T Nishino; J Nagai; H Katsuki
Journal:  J Biochem       Date:  1987-02       Impact factor: 3.387

9.  Apparent densities of spermatozoa of various mammalian species.

Authors:  S Oshio
Journal:  Gamete Res       Date:  1988-06
  9 in total
  4 in total

1.  Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: application to the enzymatic formation of cholesta-5,8-dien-3 beta-ol.

Authors:  B Ruan; J Shey; N Gerst; W K Wilson; G J Schroepfer
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-15       Impact factor: 11.205

2.  Cholesterol biosynthesis from lanosterol: development of a novel assay method and characterization of rat liver microsomal lanosterol delta 24-reductase.

Authors:  S H Bae; Y K Paik
Journal:  Biochem J       Date:  1997-09-01       Impact factor: 3.857

Review 3.  Molecular changes and signaling events occurring in spermatozoa during epididymal maturation.

Authors:  M G Gervasi; P E Visconti
Journal:  Andrology       Date:  2017-03       Impact factor: 3.842

Review 4.  Sterol regulation of developmental and oncogenic Hedgehog signaling.

Authors:  Vikas Daggubati; David R Raleigh; Navdar Sever
Journal:  Biochem Pharmacol       Date:  2021-06-07       Impact factor: 5.858

  4 in total

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