| Literature DB >> 27783950 |
Aster H Juan1, Stan Wang2, Kyung Dae Ko3, Hossein Zare3, Pei-Fang Tsai3, Xuesong Feng3, Karinna O Vivanco4, Anthony M Ascoli3, Gustavo Gutierrez-Cruz3, Jordan Krebs5, Simone Sidoli6, Adam L Knight7, Roger A Pedersen8, Benjamin A Garcia6, Rafael Casellas5, Jizhong Zou9, Vittorio Sartorelli10.
Abstract
The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state. Published by Elsevier Inc.Entities:
Keywords: H3K27 methylation; embryonic stem cells; polycomb proteins
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Year: 2016 PMID： 27783950 PMCID： PMC5123747 DOI： 10.1016/j.celrep.2016.09.087
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423