Literature DB >> 2777370

Cloning and expression of Staphylococcus saprophyticus urease gene sequences in Staphylococcus carnosus and contribution of the enzyme to virulence.

S Gatermann1, R Marre.   

Abstract

The urease gene of Staphylococcus saprophyticus CCM883 was cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned DNA sequences revealed six polypeptides (of 70, 47, 29, 27, 20, and 17 kilodaltons) that were associated with enzyme activity. Introduction of the cloned genes into a urease-negative mutant of S. saprophyticus restored the virulence of this strain, confirming our previous suggestion (S. Gatermann, J. John, and R. Marre, Infect. Immun. 57:110-116, 1989) that this enzyme is a major virulence factor of the organism and contributes mainly to cystopathogenicity.

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Year:  1989        PMID： 2777370      PMCID： PMC260761          DOI： 10.1128/iai.57.10.2998-3002.1989

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  26 in total

Review 1.  The ecology and pathogenicity of urease-producing bacteria in the urinary tract.

Authors:  R J McLean; J C Nickel; K J Cheng; J W Costerton
Journal:  Crit Rev Microbiol       Date:  1988       Impact factor: 7.624

2.  Hemagglutinating and adherence properties of Staphylococcus saprophyticus: epidemiology and virulence in experimental urinary tract infection of rats.

Authors:  S Gatermann; R Marre; J Heesemann; W Henkel
Journal:  FEMS Microbiol Immunol       Date:  1988-12

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Urease. The primary cause of infection-induced urinary stones.

Authors:  D P Griffith; D M Musher; C Itin
Journal:  Invest Urol       Date:  1976-03

5.  The ureases of Proteus strains in relation to virulence for the urinary tract.

Authors:  B W Senior; N C Bradford; D S Simpson
Journal:  J Med Microbiol       Date:  1980-11       Impact factor: 2.472

6.  Proteus mirabilis urease: genetic organization, regulation, and expression of structural genes.

Authors:  B D Jones; H L Mobley
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

7.  Proteus mirabilis urease. Partial purification and inhibition by boric acid and boronic acids.

Authors:  J M Breitenbach; R P Hausinger
Journal:  Biochem J       Date:  1988-03-15       Impact factor: 3.857

8.  Multiple proteins encoded within the urease gene complex of Proteus mirabilis.

Authors:  S E Walz; S K Wray; S I Hull; R A Hull
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490

9.  Cloning of urease gene sequences from Providencia stuartii.

Authors:  H L Mobley; B D Jones; A E Jerse
Journal:  Infect Immun       Date:  1986-10       Impact factor: 3.441

10.  Contribution of cloned virulence factors from uropathogenic Escherichia coli strains to nephropathogenicity in an experimental rat pyelonephritis model.

Authors:  R Marre; J Hacker; W Henkel; W Goebel
Journal:  Infect Immun       Date:  1986-12       Impact factor: 3.441
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  26 in total

1.  Sequence of the Klebsiella aerogenes urease genes and evidence for accessory proteins facilitating nickel incorporation.

Authors:  S B Mulrooney; R P Hausinger
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

Review 2.  Survival of Helicobacter pylori in gastric acidic territory.

Authors:  Shamshul Ansari; Yoshio Yamaoka
Journal:  Helicobacter       Date:  2017-04-12       Impact factor: 5.753

3.  The surface-associated protein of Staphylococcus saprophyticus is a lipase.

Authors:  Türkan Sakinc; Magdalena Woznowski; Michael Ebsen; Sören G Gatermann
Journal:  Infect Immun       Date:  2005-10       Impact factor: 3.441

4.  Staphylococcus saprophyticus surface-associated protein (Ssp) is associated with lifespan reduction in Caenorhabditis elegans.

Authors:  Florian Szabados; Amelie Mohner; Britta Kleine; Sören G Gatermann
Journal:  Virulence       Date:  2013-08-06       Impact factor: 5.882

5.  The hemagglutinin of Staphylococcus saprophyticus is a major adhesin for uroepithelial cells.

Authors:  H G Meyer; U Wengler-Becker; S G Gatermann
Journal:  Infect Immun       Date:  1996-09       Impact factor: 3.441

6.  Identification and characterization of a surface-associated protein (Ssp) of Staphylococcus saprophyticus.

Authors:  S Gatermann; B Kreft; R Marre; G Wanner
Journal:  Infect Immun       Date:  1992-03       Impact factor: 3.441

7.  Threonine is present instead of cysteine at the active site of urease from Staphylococcus xylosus.

Authors:  J Jose; U K Schäfer; H Kaltwasser
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552

8.  Global gene expression in Staphylococcus aureus biofilms.

Authors:  Karen E Beenken; Paul M Dunman; Fionnuala McAleese; Daphne Macapagal; Ellen Murphy; Steven J Projan; Jon S Blevins; Mark S Smeltzer
Journal:  J Bacteriol       Date:  2004-07       Impact factor: 3.490

9.  Staphylococcus saprophyticus hemagglutinin is a 160-kilodalton surface polypeptide.

Authors:  S Gatermann; H G Meyer; G Wanner
Journal:  Infect Immun       Date:  1992-10       Impact factor: 3.441

10.  A 4.6 kb DNA region of Rhizobium meliloti involved in determining urease and hydrogenase activities carries the structural genes for urease (ureA, ureB, ureC) interrupted by other open reading frames.

Authors:  G Miksch; W Arnold; P Lentzsch; U B Priefer; A Pühler
Journal:  Mol Gen Genet       Date:  1994-03
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