Literature DB >> 2777260

Different Hoechst 33342 and DAPI fluorescence of the human Y chromosome in bivariate flow karyotypes.

A Bernheim1, R Miglierina.   

Abstract

The staining properties of AT-specific dyes Hoechst 33342 and DAPI as revealed by Hoechst 33342/mithramycin and mithramycin/DAPI bivariate human flow karyotype patterns are different for chromosomes rich in heterochromatin. The peak corresponding to chromosome Y of a given cell line is higher on the A/T axis with mithramycin/DAPI staining than with mithramycin/Hoechst. The chromosome 1 was found slightly more fluorescent in mithramycin/DAPI than in mithramycin/Hoechst as judged by the slight displacement of its area on the Hoechst-DAPI axis. The other peaks did not show major differences. On the same flow karyotypes, chromosomal rearrangements were detected.

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Year:  1989        PMID： 2777260     DOI： 10.1007/BF00286716

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  18 in total

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Authors:  J W Gray; B Trask; G van den Engh; A Silva; C Lozes; S Grell; S Schonberg; L C Yu; M S Golbus
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Authors:  P Harris; E Boyd; B D Young; M A Ferguson-Smith
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3.  Interactions between pairs of DNA-binding dyes: results and implications of chromosome analysis.

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4.  Analysis of chromosomes from human peripheral lymphocytes by flow cytometry.

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Journal:  Cytometry       Date:  1981-05

5.  Design and operation of a dual laser chromosome sorter.

Authors:  R V Lebo; A M Bastian
Journal:  Cytometry       Date:  1982-11

6.  High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.

Authors:  B D Young; M A Ferguson-Smith; R Sillar; E Boyd
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

7.  High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11.

Authors:  R V Lebo; F Gorin; R J Fletterick; F T Kao; M C Cheung; B D Bruce; Y W Kan
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8.  Direct hybridization of sorted human chromosomes: localization of the Y chromosome on the flow karyotype.

Authors:  A Bernheim; P Metezeau; G Guellaën; M Fellous; M E Goldberg; R Berger
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205

9.  Quantitative karyotyping of human chromosomes by dual beam flow cytometry.

Authors:  R G Langlois; L C Yu; J W Gray; A V Carrano
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

10.  Optical Studies of the interaction of 4'-6'-diamidino-2-phenylindole with DNA and metaphase chromosomes.

Authors:  M S Lin; D E Comings; O S Alfi
Journal:  Chromosoma       Date:  1977-03-07       Impact factor: 4.316
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  2 in total

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Authors:  I Gryczynski; J R Lakowicz
Journal:  J Fluoresc       Date:  1994-12       Impact factor: 2.217

2.  Chromosome Analysis Using Benchtop Flow Analysers and High Speed Cell Sorters.

Authors:  Bee L Ng; Beiyuan Fu; Jennifer Graham; Christopher Hall; Sam Thompson
Journal:  Cytometry A       Date:  2018-12-17       Impact factor: 4.355
  2 in total

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