Literature DB >> 6950411

High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.

B D Young, M A Ferguson-Smith, R Sillar, E Boyd.   

Abstract

A method for high-resolution analysis of the human karyotype by flow cytometry has been developed. Metaphase chromosomes are prepared from short-term peripheral blood cultures, stained with ethidium bromide, and analyzed on a standard fluorescence-activated cell sorter (FACS-II). Flow karyotypes with up to 20 peaks can be obtained with coefficients of variation in the range 1-2%. At this level of resolution the contribution of many of the human chromosomes can be evaluated separately. Significant and reproducible differences between normal individuals have been detected and have been correlated with differences in the centric heterochromatin of certain chromosomes as revealed in their C-banded karyotypes.

Entities:  

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Year:  1981        PMID： 6950411      PMCID： PMC349343          DOI： 10.1073/pnas.78.12.7727

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  8 in total

1.  Chromosome measurement and sorting by flow systems.

Authors:  J W Gray; A V Carrano; L L Steinmetz; M A Van Dilla; D H Moore; B H Mayall; M L Mendelsohn
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

2.  Slit-scan flow cytometry of mammalian chromosomes.

Authors:  J W Gray; D Peters; J T Merrill; R Martin; M A Van Dilla
Journal:  J Histochem Cytochem       Date:  1979-01       Impact factor: 2.479

3.  Variability of the time at which PHA-stimulated lymphocytes initiate DNA synthesis.

Authors:  L Sörén
Journal:  Exp Cell Res       Date:  1973-03-30       Impact factor: 3.905

4.  A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH.

Authors:  W Wray; E Stubblefield
Journal:  Exp Cell Res       Date:  1970-03       Impact factor: 3.905

5.  A new method for the preparation of metaphase chromosomes for flow analysis.

Authors:  R Sillar; B D Young
Journal:  J Histochem Cytochem       Date:  1981-01       Impact factor: 2.479

6.  Measurement and purification of human chromosomes by flow cytometry and sorting.

Authors:  A V Carrano; J W Gray; R G Langlois; K J Burkhart-Schultz; M A Van Dilla
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

7.  Fluorescence polarization and pulse width analysis of chromosomes by a flow system.

Authors:  L S Cram; D J Arndt-Jovin; B G Grimwade; T M Jovin
Journal:  J Histochem Cytochem       Date:  1979-01       Impact factor: 2.479

8.  Frequency of satellite association of human chromosomes is correlated with amount of Ag-staining of the nucleolus organizer region.

Authors:  D A Miller; R Tantravahi; V G Dev; O J Miller
Journal:  Am J Hum Genet       Date:  1977-09       Impact factor: 11.025
  8 in total
  40 in total

1.  Technical improvements for flow karyotyping by standard FACS 440 flow cytometer.

Authors:  M Vitale; L Zamai; A Antonucci; P Del Grande; S Papa
Journal:  Cytotechnology       Date:  1991-03       Impact factor: 2.058

2.  Introduction: chromosomes go with the flow.

Authors:  Jaroslav Dolezel; Nigel Carter; Malcolm Ferguson-Smith
Journal:  Chromosome Res       Date:  2004       Impact factor: 5.239

3.  Construction, arraying, and high-density screening of large insert libraries of human chromosomes X and 21: their potential use as reference libraries.

Authors:  D Nizetić; G Zehetner; A P Monaco; L Gellen; B D Young; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

4.  Multiple dispersed spontaneous mutations: a novel pathway of mutation in a malignant human cell line.

Authors:  J Harwood; A Tachibana; M Meuth
Journal:  Mol Cell Biol       Date:  1991-06       Impact factor: 4.272

5.  Monoparametric models of flow cytometric karyotypes with spreadsheet software.

Authors:  J Conia; P Muller; S Brown; C Bergounioux; P Gadal
Journal:  Theor Appl Genet       Date:  1989-02       Impact factor: 5.699

6.  Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line.

Authors:  S Fukushige; K Matsubara; M Yoshida; M Sasaki; T Suzuki; K Semba; K Toyoshima; T Yamamoto
Journal:  Mol Cell Biol       Date:  1986-03       Impact factor: 4.272

7.  Optimising human chromosome separation for the production of chromosome-specific DNA libraries by flow sorting.

Authors:  P Harris; E Boyd; M A Ferguson-Smith
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

8.  Application of flow karyotyping in prenatal detection of chromosome aberrations.

Authors:  J W Gray; B Trask; G van den Engh; A Silva; C Lozes; S Grell; S Schonberg; L C Yu; M S Golbus
Journal:  Am J Hum Genet       Date:  1988-01       Impact factor: 11.025

9.  Antibody labelling and flow cytometric analysis of metaphase chromosomes reveals two discrete structural forms.

Authors:  B M Turner; A Keohane
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

10.  The potential of family flow karyotyping for the detection of chromosome abnormalities.

Authors:  P Harris; A Cooke; E Boyd; B D Young; M A Ferguson-Smith
Journal:  Hum Genet       Date:  1987-06       Impact factor: 4.132
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