Nasser Razmaraii1, Hossein Babaei2, Alireza Mohajjel Nayebi3, Gholamreza Assadnassab4, Javad Ashrafi Helan5, Yadollah Azarmi3. 1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran.; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, 5166614756, Iran. 2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran.; School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, Iran. 3. School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 5166414766, Iran. 4. Department of Clinical Sciences, Tabriz Branch, Islamic Azad University, Tabriz, 5157944533, Iran. 5. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, 5166617564, Iran.
Abstract
Purpose: The aim of the present study was to determine the ability of grape seed extract (GSE) as a powerful antioxidant in preventing adverse effect of doxorubicin (DOX) on heart function. Methods: Male rats were divided into three groups: control, DOX (2 mg/kg/48h, for 12 days) and GSE (100 mg/kg/24h, for 16 days) plus DOX. Left ventricular (LV) function and hemodynamic parameters were assessed using echocardiography, electrocardiography and a Millar pressure catheter. Histopathological analysis and in vitro antitumor activity were also evaluated. Results: DOX induced heart damage in rats through decreasing the left ventricular systolic and diastolic pressures, rate of rise/decrease of LV pressure, ejection fraction, fractional shortening and contractility index as demonstrated by echocardiography, electrocardiography and hemodynamic parameters relative to control group. Our data demonstrated that GSE treatment markedly attenuated DOX-induced toxicity, structural changes in myocardium and improved ventricular function. Additionally, GSE did not intervene with the antitumor effect of DOX. Conclusion: Collectively, the results suggest that GSE is potentially protective against DOX-induced toxicity in rat heart and maybe increase therapeutic index of DOX in human cancer treatment.
Purpose: The aim of the present study was to determine the ability of grape seed extract (GSE) as a powerful antioxidant in preventing adverse effect of doxorubicin (DOX) on heart function. Methods: Male rats were divided into three groups: control, DOX (2 mg/kg/48h, for 12 days) and GSE (100 mg/kg/24h, for 16 days) plus DOX. Left ventricular (LV) function and hemodynamic parameters were assessed using echocardiography, electrocardiography and a Millar pressure catheter. Histopathological analysis and in vitro antitumor activity were also evaluated. Results:DOX induced heart damage in rats through decreasing the left ventricular systolic and diastolic pressures, rate of rise/decrease of LV pressure, ejection fraction, fractional shortening and contractility index as demonstrated by echocardiography, electrocardiography and hemodynamic parameters relative to control group. Our data demonstrated that GSE treatment markedly attenuated DOX-induced toxicity, structural changes in myocardium and improved ventricular function. Additionally, GSE did not intervene with the antitumor effect of DOX. Conclusion: Collectively, the results suggest that GSE is potentially protective against DOX-induced toxicity in rat heart and maybe increase therapeutic index of DOX in humancancer treatment.
Authors: Joffrey Ducroq; H Moha ou Maati; S Guilbot; S Dilly; E Laemmel; C Pons-Himbert; J F Faivre; P Bois; O Stücker; M Le Grand Journal: Br J Pharmacol Date: 2009-08-24 Impact factor: 8.739