Antonella Antonelli1, Carla Sfara1, Oliver Weber2, Ulrich Pison3, Elisabetta Manuali4, Sonia Salamida4, Mauro Magnani1. 1. Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via Saffi 2, 61029 Urbino (PU), Italy. 2. Philips Medical Systems DMC GmbH, Röntgenstraβe 24-26, D-22335 Hamburg, Germany. 3. Charité-Universitätsmedizin Berlin, CC7, Augustenburger Platz 1, 13353 Berlin, Germany. 4. Laboratory of Histopathology and Clinical Chemistry, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Salvemini 1, 06126 Perugia (PG), Italy.
Abstract
AIM: The biomedical application of contrast agents based on superparamagnetic iron oxide nanoparticles is still limited because of their short intravascular half-life. The potential of red blood cells (RBCs) loaded with new ferucarbotran nanoparticles as magnetic contrast agents with longer blood retention time has been investigated. MATERIALS & METHODS: Ferucarbotran was loaded into RBCs by a procedure of hypotonic dialysis and isotonic resealing. Ferucarbotran amounts encapsulated in RBCs were determined by NMR. The survival of ferucarbotran-loaded RBCs and bulk ferucarbotran was evaluated in the mouse bloodstream. RESULTS: Blood retention time of these RBC constructs is longer (∼14 days) than the bulk ferucarbotran (∼1 h) with a slower Fe clearance from liver and spleen. CONCLUSION: Ferucarbotran-loaded RBCs could be used as potential contrasting agents for diagnostic applications in MRI/magnetic particle imaging.
AIM: The biomedical application of contrast agents based on superparamagnetic iron oxide nanoparticles is still limited because of their short intravascular half-life. The potential of red blood cells (RBCs) loaded with new ferucarbotran nanoparticles as magnetic contrast agents with longer blood retention time has been investigated. MATERIALS & METHODS:Ferucarbotran was loaded into RBCs by a procedure of hypotonic dialysis and isotonic resealing. Ferucarbotran amounts encapsulated in RBCs were determined by NMR. The survival of ferucarbotran-loaded RBCs and bulk ferucarbotran was evaluated in the mouse bloodstream. RESULTS: Blood retention time of these RBC constructs is longer (∼14 days) than the bulk ferucarbotran (∼1 h) with a slower Fe clearance from liver and spleen. CONCLUSION:Ferucarbotran-loaded RBCs could be used as potential contrasting agents for diagnostic applications in MRI/magnetic particle imaging.
Entities:
Keywords:
ferucarbotran; magnetic particle imaging; magnetic particle spectroscopy; magnetic resonance imaging; red blood cells; superparamagnetic iron oxides
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